| Iron transport in cancer cell culture suspensions measured by cell magnetophoresis. | |
| | |
MedLine Citation:
|
PMID: 22500468 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Cell motion in a magnetic field reveals the presence of intracellular paramagnetic elements, such as iron or manganese. Under controlled field and liquid media composition, such motion previously allowed us to compare the paramagnetic contribution to cell magnetic susceptibility in erythrocytes differing in the spin state of heme associated with hemoglobin. The method is now tested on cells with less obvious paramagnetic properties: cell cultures derived from human cancers to determine if the magnetophoretic mobility (MM) measurement is sufficiently sensitive to the dysregulation of the intracellular iron metabolism as suggested by reports on loss of iron homeostasis in cancer. The cell lines included hepatocellular carcinoma (Hep 3B 2.1-7 and Hep G2), promyelocytic (HL-60) and chronic myelogenous (K-562) leukemias, histiocytic lymphoma (U-937), tongue (CAL 27) and pharyngeal (Detroit 562) carcinomas, and epitheloid carcinoma (HeLa), whose MM was measured in complete media with standard and elevated soluble iron (ferric nitrate and ferric ammonium citrate), against oxy- and met-hemoglobin erythrocytes used as controls. Different cell lines responded differently to the magnetic field and the soluble iron concentrations in culture media establishing the possibility of single cell elemental analysis by magnetophoresis and magnetic cell separation based upon differences in intracellular iron concentration. |
| | |
Authors:
|
Xiaoxia Jin; Jeffrey J Chalmers; Maciej Zborowski |
Related Documents
:
|
21241168 - Metabolomic analysis of resveratrol-induced effects in the human breast cancer cell lin... 374628 - Progress report of the tudab project for automated cancer cell detection. 2393798 - Fractionated urinary cytology in the follow-up of bladder cancer. 2415648 - Characterization of human pericardial macrophages. 19067588 - Identification of three regimes of behavior for cell attachment on topographically patt... 16827158 - Gl331, a topoisomerase ii inhibitor, induces radiosensitization of human glioma cells. |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural Date: 2012-04-26 |
Journal Detail:
|
Title: Analytical chemistry Volume: 84 ISSN: 1520-6882 ISO Abbreviation: Anal. Chem. Publication Date: 2012 May |
Date Detail:
|
Created Date: 2012-05-15 Completed Date: 2012-09-19 Revised Date: 2013-05-17 |
Medline Journal Info:
|
Nlm Unique ID: 0370536 Medline TA: Anal Chem Country: United States |
Other Details:
|
Languages: eng Pagination: 4520-6 Citation Subset: IM |
Affiliation:
|
Department of Biomedical Engineering/ND20, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio 44195, United States. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Antigens, CD
/
metabolism Biological Transport Cell Line, Tumor Cell Separation Ferritins / metabolism Flow Cytometry HL-60 Cells HeLa Cells Hemoglobins / chemistry* Hep G2 Cells Humans Iron / analysis* K562 Cells Magnetic Fields Manganese / analysis Mass Spectrometry* Receptors, Transferrin / metabolism |
| Grant Support | |
ID/Acronym/Agency:
|
R01 CA062349/CA/NCI NIH HHS; R01 CA62349/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Antigens, CD; 0/CD71 antigen; 0/Hemoglobins; 0/Receptors, Transferrin; 7439-89-6/Iron; 7439-96-5/Manganese; 9007-73-2/Ferritins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Immunoreagent generation and competitive assay development for cyprodinil analysis.
Next Document: A review on decision support for massive transfusion: understanding human factors to support the imp...