Document Detail

Iron transport in cancer cell culture suspensions measured by cell magnetophoresis.
MedLine Citation:
PMID:  22500468     Owner:  NLM     Status:  MEDLINE    
Cell motion in a magnetic field reveals the presence of intracellular paramagnetic elements, such as iron or manganese. Under controlled field and liquid media composition, such motion previously allowed us to compare the paramagnetic contribution to cell magnetic susceptibility in erythrocytes differing in the spin state of heme associated with hemoglobin. The method is now tested on cells with less obvious paramagnetic properties: cell cultures derived from human cancers to determine if the magnetophoretic mobility (MM) measurement is sufficiently sensitive to the dysregulation of the intracellular iron metabolism as suggested by reports on loss of iron homeostasis in cancer. The cell lines included hepatocellular carcinoma (Hep 3B 2.1-7 and Hep G2), promyelocytic (HL-60) and chronic myelogenous (K-562) leukemias, histiocytic lymphoma (U-937), tongue (CAL 27) and pharyngeal (Detroit 562) carcinomas, and epitheloid carcinoma (HeLa), whose MM was measured in complete media with standard and elevated soluble iron (ferric nitrate and ferric ammonium citrate), against oxy- and met-hemoglobin erythrocytes used as controls. Different cell lines responded differently to the magnetic field and the soluble iron concentrations in culture media establishing the possibility of single cell elemental analysis by magnetophoresis and magnetic cell separation based upon differences in intracellular iron concentration.
Xiaoxia Jin; Jeffrey J Chalmers; Maciej Zborowski
Related Documents :
20228178 - Hsp-mediated cytoprotection of mesothelial cells in experimental acute peritoneal dialy...
21190228 - Inhibition of acid ceramidase by a 2-substituted aminoethanol amide synergistically sen...
2393798 - Fractionated urinary cytology in the follow-up of bladder cancer.
1523928 - Cytobrush and wooden spatula for oral exfoliative cytology. a comparison.
3909208 - Photoreactivation of damage induced by ionizing radiation in yeast cells.
7664318 - Chemical hypoxia increases cytosolic ca2+ and oxygen free radical formation.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-04-26
Journal Detail:
Title:  Analytical chemistry     Volume:  84     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-05-15     Completed Date:  2012-09-19     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4520-6     Citation Subset:  IM    
Department of Biomedical Engineering/ND20, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio 44195, United States.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Antigens, CD / metabolism
Biological Transport
Cell Line, Tumor
Cell Separation
Ferritins / metabolism
Flow Cytometry
HL-60 Cells
HeLa Cells
Hemoglobins / chemistry*
Hep G2 Cells
Iron / analysis*
K562 Cells
Magnetic Fields
Manganese / analysis
Mass Spectrometry*
Receptors, Transferrin / metabolism
Grant Support
Reg. No./Substance:
0/Antigens, CD; 0/CD71 antigen; 0/Hemoglobins; 0/Receptors, Transferrin; 7439-89-6/Iron; 7439-96-5/Manganese; 9007-73-2/Ferritins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Immunoreagent generation and competitive assay development for cyprodinil analysis.
Next Document:  A review on decision support for massive transfusion: understanding human factors to support the imp...