Document Detail

Iron resistance of hepatic lesions and nephroblastoma in rainbow trout (Salmo gairdneri) exposed to MNNG.
MedLine Citation:
PMID:  2554478     Owner:  NLM     Status:  MEDLINE    
Histochemical markers are important for the early detection of chemically initiated neoplasia in experimental animal studies. The marker, iron resistance, was evaluated in the Shasta strain of rainbow trout (Salmo gairdneri). Twenty-one-day-old trout embryos were exposed to 100 ppm aqueous N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) for 30 min in a static water bath. Fish were fed a semipurified diet, and sampled monthly from the 4th to the 9th month. Two days before sampling, fish were iron-loaded with a single ip dose of 0.30 mg iron dextran/100 g body weight. Livers and kidneys were conventionally processed to paraffin sections for iron, or hematoxylin and eosin (H&E) staining. Normal hepatocytes accumulated iron in pericanalicular locations, but in hepatocytes from carcinogen-altered foci and tumors, iron staining was clearly reduced or absent. Normal renal tubule cells exhibited slight to moderate iron staining, while those from nephroblastoma were iron resistant. These results establish iron resistance as a property of preneoplastic and neoplastic trout hepatocytes and nephroblastoma cells for the first time. Iron resistance may offer a practical histochemical marker in experimental fish models of hepatocellular carcinoma and nephroblastoma.
B C Lee; J D Hendricks; G S Bailey
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Toxicologic pathology     Volume:  17     ISSN:  0192-6233     ISO Abbreviation:  Toxicol Pathol     Publication Date:  1989  
Date Detail:
Created Date:  1989-12-21     Completed Date:  1989-12-21     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  7905907     Medline TA:  Toxicol Pathol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  474-82     Citation Subset:  IM    
Department of Food Science & Technology, Oregon State University, Corvallis 97331-6602.
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MeSH Terms
Adenoma / chemically induced,  metabolism
Drug-Induced Liver Injury / metabolism*
Iron / diagnostic use*
Kidney Neoplasms / chemically induced*,  metabolism
Liver Neoplasms, Experimental / chemically induced,  metabolism
Methylnitronitrosoguanidine / toxicity*
Salmonidae / physiology*
Trout / physiology*
Tumor Markers, Biological
Wilms Tumor / chemically induced*,  metabolism
Grant Support
Reg. No./Substance:
0/Tumor Markers, Biological; 70-25-7/Methylnitronitrosoguanidine; 7439-89-6/Iron

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