Document Detail


Iron release from macrophages after erythrophagocytosis is up-regulated by ferroportin 1 overexpression and down-regulated by hepcidin.
MedLine Citation:
PMID:  15665091     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ferroportin 1 (FPN1) is transmembrane protein involved in iron homeostasis. In the duodenum, FPN1 localizes to the basolateral surface of enterocytes where it appears to export iron out of the cell and into the portal circulation. FPN1 is also abundantly expressed in reticuloendothelial macrophages of the liver, spleen, and bone marrow, suggesting that this protein serves as an iron exporter in cells that recycle iron from senescent red blood cells. To directly test the hypothesis that FPN1 functions in the export of iron after erythrophagocytosis, FPN1 was stably expressed in J774 mouse macrophages by using retroviral transduction, and release of 59Fe after phagocytosis of 59Fe-labeled rat erythrocytes was measured. J774 cells overexpressing FPN1 released 70% more 59Fe after erythrophagocytosis than control cells, consistent with a role in the recycling of iron from senescent red cells. Treatment of cells with the peptide hormone hepcidin, a systemic regulator of iron metabolism, dramatically decreased FPN1 protein levels and significantly reduced the efflux of 59Fe after erythrophagocytosis. Subsequent fractionation of the total released 59Fe into heme and nonheme compounds revealed that hepcidin treatment reduced the release of nonheme 59Fe by 50% and 25% from control and FPN1-overexpressing cells, respectively, but did not diminish efflux of 59Fe-heme. We conclude that FPN1 is directly involved in the export of iron during erythrocyte-iron recycling by macrophages.
Authors:
Mitchell D Knutson; Mohamed Oukka; Lindsey M Koss; Fikret Aydemir; Marianne Wessling-Resnick
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.     Date:  2005-01-21
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  102     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2005-02-02     Completed Date:  2005-03-28     Revised Date:  2013-06-14    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1324-8     Citation Subset:  IM    
Affiliation:
Food Science and Human Nutrition Department, University of Florida, Gainesville, FL 32611, USA. mdknutson@ifas.ufl.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Antimicrobial Cationic Peptides / genetics*
Cation Transport Proteins / genetics*
Cell Line
Erythrocytes / physiology*
Gene Expression Regulation
Humans
Iron / metabolism*
Macrophages / physiology*
Mice
Phagocytosis / physiology*
Grant Support
ID/Acronym/Agency:
DK065064/DK/NIDDK NIH HHS; DK56160/DK/NIDDK NIH HHS; R01 DK056160/DK/NIDDK NIH HHS; R01 DK056160-01/DK/NIDDK NIH HHS; R01 DK056160-02/DK/NIDDK NIH HHS; R01 DK056160-03/DK/NIDDK NIH HHS; R01 DK056160-04/DK/NIDDK NIH HHS; R01 DK056160-05/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Antimicrobial Cationic Peptides; 0/Cation Transport Proteins; 0/hepcidin; 0/metal transporting protein 1; 7439-89-6/Iron
Comments/Corrections

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