| Iron-related proteins: candidate urine biomarkers in childhood HIV-associated renal diseases. | |
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MedLine Citation:
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PMID: 19279121 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Because of the risk of performing renal biopsies in children with co-morbid conditions, we carried out this study to identify candidate protein biomarkers in the urine of HIV-infected children with renal disease. Design, setting, participants & measurements: Urine samples from HIV-infected children with biopsy proven HIV-nephropathy (HIVAN; n = 4), HIV-associated Hemolytic Uremic Syndrome (HIV-HUS; n = 2), or no renal disease (n = 3) were analyzed by two-dimensional electrophoresis (2-DE) and proteomic methods. Positive findings were confirmed in HIV-infected children with (n = 20) and without (n = 10) proteinuria using commercially available assays. RESULTS: By 2-DE analysis, a single urine marker was not sufficient to distinguish children with HIVAN from the others. High urine levels of beta(2)-microglobulin and retinol-binding protein (RBP) suggested the presence of tubular injury. In addition, we found elevated urine levels of iron and the iron-related proteins, transferrin, hemopexin, haptoglobin, lactoferrin, and neutrophil gelatinase-associated lipocalin (NGAL), in children with HIVAN and HIV-HUS. Furthermore, we detected a significant accumulation of iron in the urine and kidneys of HIV-transgenic (Tg) rats with renal disease. CONCLUSION: These findings suggest that iron and iron-related proteins might be promising candidate urine biomarkers to identify HIV-infected children at risk of developing HIVAN and HIV-HUS. Moreover, based on the results of previous studies, we speculate that the release or accumulation of iron in the kidney of HIV-infected children may contribute to the rapid progression of their renal disease, and could become a new therapeutic target against HIVAN and HIV-HUS. |
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Authors:
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Angel A Soler-García; Douglas Johnson; Yetrib Hathout; Patricio E Ray |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2009-03-11 |
Journal Detail:
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Title: Clinical journal of the American Society of Nephrology : CJASN Volume: 4 ISSN: 1555-905X ISO Abbreviation: Clin J Am Soc Nephrol Publication Date: 2009 Apr |
Date Detail:
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Created Date: 2009-04-06 Completed Date: 2009-06-08 Revised Date: 2010-09-22 |
Medline Journal Info:
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Nlm Unique ID: 101271570 Medline TA: Clin J Am Soc Nephrol Country: United States |
Other Details:
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Languages: eng Pagination: 763-71 Citation Subset: IM |
Affiliation:
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Division of Nephrology, Center for Cancer and Immunology Research, Children's Research Institute, NW, Washington, DC 20010, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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AIDS-Associated Nephropathy
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pathology,
urine*,
virology Acute-Phase Proteins / urine Animals Biological Markers / urine Biopsy Blood Proteins / urine* Case-Control Studies Disease Models, Animal HIV Infections / complications, pathology, urine, virology* HIV-1 / genetics, pathogenicity* Haptoglobins / urine Hemolytic-Uremic Syndrome / pathology, urine*, virology Hemopexin / urine Humans Iron / urine Lactoferrin / urine Lipocalins / urine Predictive Value of Tests Proteinuria / urine*, virology Proto-Oncogene Proteins / urine Rats Rats, Transgenic Time Factors Transferrin / urine |
| Grant Support | |
ID/Acronym/Agency:
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5R24 HD050846/HD/NICHD NIH HHS; R01-DK049419-10S2/DK/NIDDK NIH HHS; R01-HL55605-08S1/HL/NHLBI NIH HHS; R21 NICAN-AT002278/AT/NCCAM NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Acute-Phase Proteins; 0/Biological Markers; 0/Blood Proteins; 0/Haptoglobins; 0/LCN2 protein, human; 0/Lactoferrin; 0/Lipocalins; 0/Proto-Oncogene Proteins; 11096-37-0/Transferrin; 7439-89-6/Iron; 9013-71-2/Hemopexin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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