| Iron homeostasis in astrocytes and microglia is differentially regulated by TNF-α and TGF-β1. | |
| | |
MedLine Citation:
|
PMID: 22298416 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Abnormal iron homeostasis is increasingly thought to contribute to the pathogenesis of several neurodegenerative disorders. We have previously reported impaired iron homeostasis in a mouse model of spinal cord injury and in a mouse model of amyotrophic lateral sclerosis. Both these disorders are associated with CNS inflammation. However, what effect inflammation, and in particular, inflammatory cytokines have on iron homeostasis in CNS glia remains largely unknown. Here we report that the proinflammatory cytokine TNF-α, and the anti-inflammatory cytokine TGF-β1 affect iron homeostasis in astrocytes and microglia in distinct ways. Treatment of astrocytes in vitro with TNF-α induced the expression of the iron importer "divalent iron transporter 1" (DMT1) and suppressed the expression of the iron exporter ferroportin (FPN). However, TGF-β1 had no effect on DMT1 expression but increased the expression of FPN in astrocytes. In microglia, on the other hand, both cytokines caused induction of DMT1 and suppression of FPN expression. Iron influx and efflux assays in vitro confirmed that iron homeostasis in astrocytes and microglia is differentially regulated by these cytokines. In particular, TNF-α caused an increase in iron uptake and retention by both astrocytes and microglia, while TGF-β1 promoted iron efflux from astrocytes but caused iron retention in microglia. These data suggest that these two cytokines, which are expressed in CNS inflammation in injury and disease, can have profound and divergent effects on iron homeostasis in astrocytes and microglia. © 2012 Wiley Periodicals, Inc. |
| | |
Authors:
|
Khizr I Rathore; Adriana Redensek; Samuel David |
Related Documents
:
|
15152266 - Cytosine arabinoside induces costimulatory molecule expression in acute myeloid leukemi... 18393856 - Anti-infective and anti-tumor agents based on the depletion of immune suppressive effects. 22423966 - Autophagy protein rubicon mediates phagocytic nadph oxidase activation in response to m... 6299906 - An assessment of sequential measurements of immune complex levels in ovarian cancer pat... 1588466 - Killing of paracoccidioides brasiliensis conidia by pulmonary macrophages and the effec... 9056426 - Altered gene expression associated with apoptosis in a pre-b-leukemic cell line followi... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-2-1 |
Journal Detail:
|
Title: Glia Volume: - ISSN: 1098-1136 ISO Abbreviation: - Publication Date: 2012 Feb |
Date Detail:
|
Created Date: 2012-2-2 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8806785 Medline TA: Glia Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2012 Wiley Periodicals, Inc. |
Affiliation:
|
Center for Research in Neuroscience, The Research Institute of the McGill University Health Center, Montreal, Quebec, Canada. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Protective Effect of Brazilian Propolis Against Hepatic Oxidative Damage in Rats with Water-immersio...
Next Document: Online Cognitive-Behavioural Treatment of Bulimic Symptoms: A Randomized Controlled Trial.