| Iron homeostasis affects antibiotic-mediated cell death in Pseudomonas species. | |
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MedLine Citation:
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PMID: 20479007 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Antibiotics can induce cell death via a variety of action modes, including the inhibition of transcription, ribosomal function, and cell wall biosynthesis. In this study, we demonstrated directly that iron availability is important to the action of antibiotics, and the ferric reductases of Pseudomonas putida and Pseudomonas aeruginosa could accelerate antibiotic-mediated cell death by promoting the Fenton reaction. The modulation of reduced nicotinamide-adenine dinucleotide (NADH) levels and iron chelation affected the actions of antibiotics. Interestingly, the deletion of the ferric reductase gene confers more antibiotic resistance upon cells, and its overexpression accelerates antibiotic-mediated cell death. The results of transcriptome analysis showed that both Pseudomonas species induce many oxidative stress genes under antibiotic conditions, which could not be observed in ferric reductase mutants. Our results indicate that iron homeostasis is crucial for bacterial cell survival under antibiotics and should constitute a significant target for boosting the action of antibiotics. |
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Authors:
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Jinki Yeom; James A Imlay; Woojun Park |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-05-17 |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 285 ISSN: 1083-351X ISO Abbreviation: J. Biol. Chem. Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-07-12 Completed Date: 2010-08-06 Revised Date: 2011-08-01 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
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Languages: eng Pagination: 22689-95 Citation Subset: IM |
Affiliation:
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Division of Environmental Science and Ecological Engineering, Korea University, Seoul, 136-713, South Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Anti-Bacterial Agents
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pharmacology* Biological Markers / metabolism DNA Damage Gene Expression Profiling Gene Expression Regulation, Bacterial / drug effects Homeostasis / drug effects*, genetics Intracellular Space / drug effects, metabolism Iron / metabolism* Microbial Sensitivity Tests Microbial Viability / drug effects* Oxidative Stress / genetics Pseudomonas aeruginosa / cytology*, drug effects, genetics Pseudomonas putida / cytology*, drug effects, genetics Stress, Physiological / drug effects, genetics |
| Chemical | |
Reg. No./Substance:
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0/Anti-Bacterial Agents; 0/Biological Markers; 7439-89-6/Iron |
| Comments/Corrections | |
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