| Iron, Glucose and Intrinsic Factors Alter Sphingolipid Composition as Yeast Cells Enter Stationary Phase. | |
| | |
MedLine Citation:
|
PMID: 23286903 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Survival of Saccharomyces cerevisiae cells, like most microorganisms, requires switching from a rapidly dividing to a non-dividing or stationary state. To further understand how cells navigate this switch, we examined sphingolipids since they are key structural elements of membranes and also regulate signaling pathways vital for survival. During and after the switch to a non-dividing state there is a large increase in total free and sphingolipid-bound long chain-bases and an even larger increase in free and bound C20-long-chain bases, which are nearly undetectable in dividing cells. These changes are due to intrinsic factors including Orm1 and Orm2, ceramide synthase, Lcb4 kinase and the Tsc3 subunit of serine palmitoyltransferase as well as extrinsic factors including glucose and iron. Lowering the concentration of glucose, a form of calorie restriction, decreases the level of LCBs, which is consistent with the idea that reducing the level of some sphingolipids enhances lifespan. In contrast, iron deprivation increases LCB levels and decreases long term survival; however, these phenomena may not be related because iron deprivation disrupts many metabolic pathways. The correlation between increased LCBs and shorter lifespan is unsupported at this time. The physiological rise in LCBs that we observe may serve to modulate nutrient transporters and possibly other membrane phenomena that contribute to enhanced stress resistance and survival in stationary phase. |
| | |
Authors:
|
Robert L Lester; Bradley R Withers; Megan A Schultz; Robert C Dickson |
Related Documents
:
|
19833543 - Non-genetic cell-to-cell variability and the consequences for pharmacology. 19846663 - Exploring the roles of diaphanous and enabled activity in shaping the balance between f... 7812943 - Long-term persistence and cytokinetics of human tumor cells in vitro following high-dos... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-12-31 |
Journal Detail:
|
Title: Biochimica et biophysica acta Volume: - ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 2012 Dec |
Date Detail:
|
Created Date: 2013-1-4 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2012. Published by Elsevier B.V. |
Affiliation:
|
Department of Molecular and Cellular Biochemistry and the Lucille Markey Cancer Center, University of Kentucky College of Medicine, Lexington, Kentucky, 40536, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Melanopsin, Photosensitive Ganglion Cells, and Seasonal Affective Disorder.
Next Document: Evidence-based Toxicity Evaluation and Scheduling of Chinese Herbal Medicines.