Document Detail


Iron, glucose and intrinsic factors alter sphingolipid composition as yeast cells enter stationary phase.
MedLine Citation:
PMID:  23286903     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Survival of Saccharomyces cerevisiae cells, like most microorganisms, requires switching from a rapidly dividing to a non-dividing or stationary state. To further understand how cells navigate this switch, we examined sphingolipids since they are key structural elements of membranes and also regulate signaling pathways vital for survival. During and after the switch to a non-dividing state there is a large increase in total free and sphingolipid-bound long chain-bases and an even larger increase in free and bound C20-long-chain bases, which are nearly undetectable in dividing cells. These changes are due to intrinsic factors including Orm1 and Orm2, ceramide synthase, Lcb4 kinase and the Tsc3 subunit of serine palmitoyltransferase as well as extrinsic factors including glucose and iron. Lowering the concentration of glucose, a form of calorie restriction, decreases the level of LCBs, which is consistent with the idea that reducing the level of some sphingolipids enhances lifespan. In contrast, iron deprivation increases LCB levels and decreases long term survival; however, these phenomena may not be related because iron deprivation disrupts many metabolic pathways. The correlation between increased LCBs and shorter lifespan is unsupported at this time. The physiological rise in LCBs that we observe may serve to modulate nutrient transporters and possibly other membrane phenomena that contribute to enhanced stress resistance and survival in stationary phase.
Authors:
Robert L Lester; Bradley R Withers; Megan A Schultz; Robert C Dickson
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-12-31
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1831     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-03-04     Completed Date:  2013-06-11     Revised Date:  2014-04-02    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  726-36     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 Elsevier B.V. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Cell Cycle
Glucose / metabolism*
Intrinsic Factor / metabolism*
Iron / metabolism*
Saccharomyces cerevisiae / cytology,  metabolism*
Saccharomyces cerevisiae Proteins / metabolism*
Sphingolipids / metabolism*
Grant Support
ID/Acronym/Agency:
AG024377/AG/NIA NIH HHS; R01 AG024377/AG/NIA NIH HHS
Chemical
Reg. No./Substance:
0/Saccharomyces cerevisiae Proteins; 0/Sphingolipids; 9008-12-2/Intrinsic Factor; E1UOL152H7/Iron; IY9XDZ35W2/Glucose
Comments/Corrections

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