Document Detail

IpgD, a protein secreted by the type III secretion machinery of Shigella flexneri, is chaperoned by IpgE and implicated in entry focus formation.
MedLine Citation:
PMID:  11029686     Owner:  NLM     Status:  MEDLINE    
Invasion of epithelial cells by Shigella flexneri involves entry and intercellular dissemination. Entry of bacteria into non-phagocytic cells requires the IpaA-D proteins that are secreted by the Mxi-Spa type III secretion machinery. Type III secretion systems are found in several Gram-negative pathogens and serve to inject bacterial effector proteins directly into the cytoplasm of host cells. In this study, we have analysed the IpgD protein of S. flexneri, the gene of which is located on the virulence plasmid at the 5' end of the mxi-spa locus. We have shown that IpgD (i) is stored in the bacterial cytoplasm in association with a specific chaperone, IpgE; (ii) is secreted by the Mxi-Spa type III secretion system in amounts similar to those of the IpaA-D proteins; (iii) is associated with IpaA in the extracellular medium; and (iv) is involved in the modulation of the host cell response after contact of the bacterium with epithelial cells. This suggests that IpgD is an effector that might be injected into host cells to manipulate cellular processes during infection.
K Niebuhr; N Jouihri; A Allaoui; P Gounon; P J Sansonetti; C Parsot
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular microbiology     Volume:  38     ISSN:  0950-382X     ISO Abbreviation:  Mol. Microbiol.     Publication Date:  2000 Oct 
Date Detail:
Created Date:  2000-11-20     Completed Date:  2000-11-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8712028     Medline TA:  Mol Microbiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  8-19     Citation Subset:  IM    
Unité de Pathogenie Microbienne Moléculaire, INSERM U389, Institut Pasteur, 28 Rue du Dr Roux, 75724 Paris Cèdex 15, France.
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MeSH Terms
Bacterial Proteins / metabolism*
Base Sequence
Culture Media
DNA Primers
Hela Cells
Membrane Fusion*
Molecular Chaperones / metabolism*
Shigella flexneri / physiology*
Reg. No./Substance:
0/Bacterial Proteins; 0/Culture Media; 0/DNA Primers; 0/Molecular Chaperones

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