Document Detail


Ionizing radiation causes active degradation and reduces matrix synthesis in articular cartilage.
MedLine Citation:
PMID:  23134087     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: Little is known regarding radiation effects on adult articular (joint) cartilage, though joint damage has been reported following cancer treatment or occupational exposures. The aim of this study was to determine if radiation can reduce cartilage matrix production, induce cartilage degradation, or interfere with the anabolic effects of IGF-1.
MATERIALS AND METHODS: Isolated chondrocytes cultured in monolayers and whole explants harvested from ankles of human donors and knees of pigs were irradiated with 2 or 10 Gy γ-rays, with or without IGF-1 stimulation. Proteoglycan synthesis and IGF-1 signaling were examined at Day 1; cartilage degradation throughout the first 96 hours.
RESULTS: Human and pig cartilage responded similarly to radiation. Cell viability was unchanged. Basal and IGF-1 stimulated proteoglycan synthesis was reduced following exposure, particularly following 10 Gy. Both doses decreased IGF-induced Akt activation and IGF-1 receptor phosphorylation. Matrix metalloproteinases (ADAMTS5, MMP-1, and MMP-13) and proteoglycans were released into media after 2 and 10 Gy.
CONCLUSIONS: Radiation induced an active degradation of cartilage, reduced proteoglycan synthesis, and impaired IGF-1 signaling in human and pig chondrocytes. Lowered Akt activation could account for decreased matrix synthesis. Radiation may cause a functional decline of cartilage health in joints after exposure, contributing to arthropathy.
Authors:
Jeffrey S Willey; David L Long; Kadie S Vanderman; Richard F Loeser
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-12-12
Journal Detail:
Title:  International journal of radiation biology     Volume:  89     ISSN:  1362-3095     ISO Abbreviation:  Int. J. Radiat. Biol.     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-04-01     Completed Date:  2013-05-21     Revised Date:  2013-10-17    
Medline Journal Info:
Nlm Unique ID:  8809243     Medline TA:  Int J Radiat Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  268-77     Citation Subset:  IM; S    
Affiliation:
Department of Radiation Oncology, Wake Forest School of Medicine, Winston-Salem, North Carolina 27106, USA. jwilley@wakehealth.edu
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MeSH Terms
Descriptor/Qualifier:
Adult
Animals
Cartilage, Articular / cytology,  drug effects,  metabolism*,  radiation effects*
Cell Survival / drug effects
Chondrocytes / cytology,  drug effects,  metabolism,  radiation effects
DNA / metabolism
Glycosaminoglycans / biosynthesis,  secretion
Humans
Insulin-Like Growth Factor I / pharmacology
Proteoglycans / biosynthesis
Signal Transduction / drug effects,  radiation effects
Swine
Grant Support
ID/Acronym/Agency:
R01 AG016697/AG/NIA NIH HHS; R01AG016697/AG/NIA NIH HHS; T32 CA113267/CA/NCI NIH HHS; T32 CA113267/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Glycosaminoglycans; 0/Proteoglycans; 67763-96-6/Insulin-Like Growth Factor I; 9007-49-2/DNA
Comments/Corrections

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