| Ionic properties of the acetylcholine receptor in cultured rat myotubes. | |
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MedLine Citation:
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PMID: 460 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The acetylcholine reversal potential (Er) of cultured rat myotubes is -3mV. When activated, the receptor is permeable to K+ and Na+, but not to Cl- ions. Measurement of Er in Tris+-substituted, Na-free medium also indicated a permeability to Tris+ ions. Unlike adult frog muscle the magnitude of Er was insensitive to change in external Ca++ (up to 30 mM) or to changes in external pH (between 6.4 and 8.9). The equivalent circuit equation describing the electrical circuit composed of two parallel ionic batteries (EK and ENa) and their respective conductances (gK and gNa), which has been generally useful in describing the Er of adult rat and frog muscle, could also be applied to rat myotubes when Er was measured over a wide range of external Na+ concentrations. The equivalent circuit equation could not be applied to myotubes bathed in media of different external K+ concentrations. In this case, the Er was more closely described by the Goldman constant field equation. Under certain circumstances, it is known that the receptor in adult rat and frog muscle can be induced to reversibly shift from behavior described by the equivalent circuit equation to that described by the Goldman equation. Attempts to similarly manipulate the responses of cultured rat myotubes were unsussessful. These trials included a reduction in temperature (15 degress C), partial alpha-bungarotoxin blodkade, and activation of responses with the cholinergic agonist, decamethonium. |
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Authors:
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A K Ritchie; D M Fambrough |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of general physiology Volume: 65 ISSN: 0022-1295 ISO Abbreviation: J. Gen. Physiol. Publication Date: 1975 Jun |
Date Detail:
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Created Date: 1976-02-13 Completed Date: 1976-02-13 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2985110R Medline TA: J Gen Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 751-67 Citation Subset: IM |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bungarotoxins / pharmacology Calcium / metabolism Cell Membrane Permeability Chlorides / metabolism* Culture Techniques Decamethonium Compounds / pharmacology Electrophysiology Hydrogen-Ion Concentration Membrane Potentials Muscles / embryology* Potassium / metabolism* Rats Receptors, Cholinergic* Sodium / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Bungarotoxins; 0/Chlorides; 0/Decamethonium Compounds; 0/Receptors, Cholinergic; 7440-09-7/Potassium; 7440-23-5/Sodium; 7440-70-2/Calcium |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): J Gen Physiol ISSN: 0022-1295 ISSN: 1540-7748 Publisher: The Rockefeller University Press |
Article Information Download PDF  Print publication date: Day: 1 Month: 6 Year: 1975 Volume: 65 Issue: 6 First Page: 751 Last Page: 767 ID: 2214888 Publisher Id: 76070881 PubMed Id: 460 |
| Ionic properties of the acetylcholine receptor in cultured rat myotubes | |
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