| Ionic currents in neuroblastoma clone E-7 cells. | |
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MedLine Citation:
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PMID: 2582319 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Ionic currents were studied in exponentially growing neuroblastoma cells (clone E-7) derived from mouse neuroblastoma C-1300 with the patch-clamp technique in the whole cell mode (Pflügers Arch., 391 (1981) 85-100). In differentiated cells, with one or several processes, an early inactivating inward current approximately equal to 50 microA/cm2 was observed in response to depolarizing steps from the holding potential -60 to -70 mV, which was insensitive to 2 microM tetrodotoxin but readily blocked by Co2+ (6 mM). This inward current was followed by a delayed outward current which was eliminated by 12 mM tetraethylammonium. In the undifferentiated cells, only delayed outward current was observed. It is suggested that, in differentiated cells both Ca2+ and delayed rectifier K+ channels exist, while only the latter are present in undifferentiated cells. |
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Authors:
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L Vyklický; J Michl; V Vlachová; L Vyklický; F Vyskocil |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Neuroscience letters Volume: 55 ISSN: 0304-3940 ISO Abbreviation: Neurosci. Lett. Publication Date: 1985 Apr |
Date Detail:
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Created Date: 1985-07-25 Completed Date: 1985-07-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7600130 Medline TA: Neurosci Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 197-201 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Calcium / metabolism Clone Cells Cobalt / pharmacology Ion Channels / physiology* Membrane Potentials* Mice Neuroblastoma Potassium / metabolism Sodium / metabolism Tetraethylammonium Tetraethylammonium Compounds / pharmacology Tetrodotoxin / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Ion Channels; 0/Tetraethylammonium Compounds; 4368-28-9/Tetrodotoxin; 66-40-0/Tetraethylammonium; 7440-09-7/Potassium; 7440-23-5/Sodium; 7440-48-4/Cobalt; 7440-70-2/Calcium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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