Document Detail


Ionic currents of human trabecular meshwork cells from control and glaucoma subjects.
MedLine Citation:
PMID:  23135060     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Increasing evidence suggests that trabecular meshwork (TM) cells participate in the regulation of intraocular pressure by controlling the rate of filtration of the aqueous humor. Ionic conductances that regulate cell volume and shape have been suggested to play an important role in TM cell volume regulation. Here, we compared ionic currents from TM cells derived from a normal subject (CTM) and from an individual affected by glaucoma (GTM). We found that while the ionic current types were similar, the current amplitudes and percentage of cells endowed with specific current at baseline were different in the two cell lines. Thus, we found that the majority of CTM cells were endowed with a swelling-activated Cl(-) current at baseline, whereas in the majority of GTM cells this current was not active at baseline and became activated only after perfusion with a hypotonic solution. An inward rectifier K(+) current was also more prevalent in CTM than in GTM cells. Our work suggests that disregulation of one or more of these ionic currents may be at the basis of TM cell participation in the development of glaucoma.
Authors:
Jeff Grant; Vu Tran; Sanjoy K Bhattacharya; Laura Bianchi
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-11-08
Journal Detail:
Title:  The Journal of membrane biology     Volume:  246     ISSN:  1432-1424     ISO Abbreviation:  J. Membr. Biol.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-01-28     Completed Date:  2013-08-08     Revised Date:  2014-02-04    
Medline Journal Info:
Nlm Unique ID:  0211301     Medline TA:  J Membr Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  167-75     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Cell Line, Tumor
Electrophysiology
Glaucoma / metabolism*
Humans
Potassium / metabolism
Potassium Channels, Inwardly Rectifying / metabolism
Trabecular Meshwork / metabolism*
Grant Support
ID/Acronym/Agency:
EY016112/EY/NEI NIH HHS; R01 NS070969/NS/NINDS NIH HHS; R01NS070969/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Potassium Channels, Inwardly Rectifying; RWP5GA015D/Potassium
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Changes in Electric Properties of Human Breast Cancer Cells.
Next Document:  Tropical storm off Myanmar coast sweeps reefs in Ritchie's Archipelago, Andaman.