Document Detail


Ion transport by amphibian antrum in vitro. I. General characteristics.
MedLine Citation:
PMID:  236668     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Both Necturus and bullfrog antrum show stable PD, resistance, and short-circuit current (Isc) when mounted in an Ussing chamber. Measurements of Na+ and Cl minus flux showed that both ions are actively transported across Necturus antrum, Na+ from secretory to nutrient, Cl minus from nutrient to secretory (both net fluxes being similar to 0.30 mueq cm minus 2 h minus 1). Only the Na+ transport contributed to the Isc and PD as evidenced by a) Na+ removal, b) the effects of amiloride on the secretory surface, c) the effects of ouabain on the nutrient side. Microelectrode experiments confirm the Na+ conductance of the secretory cell membrance, a HCO3 minus conductance of both cell membranes, and a KCl conductance across the nutrient cell membrane. In addition, antrum apparently secretes alkali (similar to 0.35 mueq cm minus 2 h minus 1), which secretion is sensitive to metabolic inhibitors and Diamox. Nutrientside HCO3 minus increased the rate of alkaline secretion and a transmucosal HCO3 minus gradient could contribute to ISC and PD. A model is proposed to account for the electrical properties of the tissue.
Authors:
G Flemström; T G Sachs
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  228     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1975 Apr 
Date Detail:
Created Date:  1975-07-18     Completed Date:  1975-07-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1188-98     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amiloride / pharmacology
Animals
Bicarbonates / metabolism
Biological Transport, Active
Cell Membrane / metabolism
Chlorides / metabolism*
Electrophysiology
Hydrogen-Ion Concentration
Microelectrodes
Models, Biological
Ouabain / pharmacology
Potassium Chloride / metabolism
Rana catesbeiana / metabolism*
Sodium / metabolism*
Stomach / metabolism*
Urodela / metabolism*
Chemical
Reg. No./Substance:
0/Bicarbonates; 0/Chlorides; 2609-46-3/Amiloride; 630-60-4/Ouabain; 7440-23-5/Sodium; 7447-40-7/Potassium Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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