Document Detail

Ion-pair reverse-phase liquid chromatographic determination of amprolium in complete feeds and premixes.
MedLine Citation:
PMID:  3384778     Owner:  NLM     Status:  MEDLINE    
Amprolium is extracted with methanol-water (2 + 1) containing 5mM dioctylsulfosuccinate (DOSS) and 10mM CaCl2. The analyte is separated from coextracted materials by isocratic ion-pair reverse-phase liquid chromatography, following removal of late-eluting materials on an acid alumina cleanup column, and is detected at 270 nm. The mobile phase contains 4mM DOSS with 0.3% diethylamine and 1% acetic acid in 40% acetonitrile. Linearity is satisfactory over the range of 2.5-50 micrograms/mL. Mean recovery, as determined by standard addition to commercial samples, is 100.1%. Accuracy was further tested in studies comparing the LC method to the official AOAC colorimetric method, using commercial samples, and was found to be satisfactory. Studies show that common poultry feed additives, grass meals, and some pelletization aids do not interfere with the analysis; however, when bentonite is present, recovery is decreased. The precision of the method, measured over several experiments on commercial samples, is satisfactory as indicated by coefficients of variation ranging from less than 1 to 4.5. A ruggedness test resulted in an overall CV of 3.2%, indicating the probable success of the method in a collaborative study.
E J Kentzer; L S Cottingham; R L Smallidge
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal - Association of Official Analytical Chemists     Volume:  71     ISSN:  0004-5756     ISO Abbreviation:  J Assoc Off Anal Chem     Publication Date:    1988 Mar-Apr
Date Detail:
Created Date:  1988-07-29     Completed Date:  1988-07-29     Revised Date:  2001-11-13    
Medline Journal Info:
Nlm Unique ID:  7505559     Medline TA:  J Assoc Off Anal Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  251-5     Citation Subset:  IM; X    
Purdue University, Department of Biochemistry, West Lafayette, IN 47907.
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MeSH Terms
Amprolium / analysis*
Animal Feed / analysis*
Chromatography, Liquid
Coccidiostats / analysis*
Food Contamination / analysis
Indicators and Reagents
Reg. No./Substance:
0/Coccidiostats; 0/Indicators and Reagents; 0/Picolines; 137-88-2/Amprolium

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