Document Detail


Ion mobility analysis of lipoprotein subfractions identifies three independent axes of cardiovascular risk.
MedLine Citation:
PMID:  19729614     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: Whereas epidemiological studies show that levels of low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) predict incident cardiovascular disease (CVD), there is limited evidence relating lipoprotein subfractions and composite measures of subfractions to risk for CVD in prospective cohort studies.
METHODS AND RESULTS: We tested whether combinations of lipoprotein subfractions independently predict CVD in a prospective cohort of 4594 initially healthy men and women (the Malmö Diet and Cancer Study, mean follow-up 12.2 years, 377 incident cardiovascular events). Plasma lipoproteins and lipoprotein subfractions were measured at baseline with a novel high-resolution ion mobility technique. Principal component analysis (PCA) of subfraction concentrations identified 3 major independent (ie, zero correlation) components of CVD risk, one representing LDL-associated risk, a second representing HDL-associated protection, and the third representing a pattern of decreased large HDL, increased small/medium LDL, and increased triglycerides. The last corresponds to the previously described "atherogenic lipoprotein phenotype." Several genes that may underlie this phenotype-CETP, LIPC, GALNT2, MLXIPL, APOA1/A5, LPL-are suggested by SNPs associated with the combination of small/medium LDL and large HDL.
CONCLUSIONS: PCA on lipoprotein subfractions yielded three independent components of CVD risk. Genetic analyses suggest these components represent independent mechanistic pathways for development of CVD.
Authors:
Kiran Musunuru; Marju Orho-Melander; Michael P Caulfield; Shuguang Li; Wael A Salameh; Richard E Reitz; Göran Berglund; Bo Hedblad; Gunnar Engström; Paul T Williams; Sekar Kathiresan; Olle Melander; Ronald M Krauss
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2009-09-03
Journal Detail:
Title:  Arteriosclerosis, thrombosis, and vascular biology     Volume:  29     ISSN:  1524-4636     ISO Abbreviation:  Arterioscler. Thromb. Vasc. Biol.     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-10-22     Completed Date:  2010-02-04     Revised Date:  2010-12-03    
Medline Journal Info:
Nlm Unique ID:  9505803     Medline TA:  Arterioscler Thromb Vasc Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1975-80     Citation Subset:  IM    
Affiliation:
Center for Human Genetic Research, Cardiology Division, Massachusetts General Hospital, Boston, MA, USA.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Biological Markers / blood
Cardiovascular Diseases / blood*
Cholesterol, HDL / blood*
Cholesterol, LDL / blood*
Chromatography, High Pressure Liquid
Chromatography, Ion Exchange
Cohort Studies
Female
Humans
Ion Transport
Lipoproteins, VLDL / blood*
Logistic Models
Male
Middle Aged
Multivariate Analysis
Predictive Value of Tests
Probability
Prognosis
Prospective Studies
Risk Assessment
Grant Support
ID/Acronym/Agency:
U01 HL069757-08/HL/NHLBI NIH HHS; U01HL069757/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Cholesterol, HDL; 0/Cholesterol, LDL; 0/Lipoproteins, VLDL

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