Document Detail


Ion-implanted polytetrafluoroethylene enhances Saccharomyces cerevisiae biofilm formation for improved immobilization.
MedLine Citation:
PMID:  22696486     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The surface of polytetrafluoroethylene (PTFE) was modified using plasma immersion ion implantation (PIII) with the aim of improving its ability to immobilize yeast. The density of immobilized cells on PIII-treated and -untreated PTFE was compared as a function of incubation time over 24 h. Rehydrated yeast cells attached to the PIII-treated PTFE surface more rapidly, with higher density, and greater attachment strength than on the untreated surface. The immobilized yeast cells were removed mechanically or chemically with sodium hydroxide and the residues left on the surfaces were analysed with Fourier transform infrared spectroscopy-attenuated total reflection (FTIR-ATR) and X-ray photoelectron spectroscopy (XPS). The results revealed that the mechanism of cell attachment on both surfaces differs and a model is presented for each. Rapid attachment on the PIII-treated surface occurs through covalent bonds of cell wall proteins and the radicals on the treated surface. In contrast, on the untreated surface, only physisorbed molecules were found in the residue and lipids were more highly concentrated than proteins. The presence of lipids in the residue was found to be a consequence of damage to the plasma membrane during the rehydration process and the increased cell stress was also apparent by the amount of Hsp12 in the protein residue. The immobilized yeast cells on PIII-treated PTFE were found to be as active as yeast cells in suspension.
Authors:
Clara T H Tran; Alexey Kondyurin; Stacey L Hirsh; David R McKenzie; Marcela M M Bilek
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-06-13
Journal Detail:
Title:  Journal of the Royal Society, Interface / the Royal Society     Volume:  9     ISSN:  1742-5662     ISO Abbreviation:  J R Soc Interface     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-09-26     Completed Date:  2013-02-11     Revised Date:  2013-11-15    
Medline Journal Info:
Nlm Unique ID:  101217269     Medline TA:  J R Soc Interface     Country:  England    
Other Details:
Languages:  eng     Pagination:  2923-35     Citation Subset:  IM    
Affiliation:
Applied and Plasma Physics (A28), University of Sydney, Sydney, New South Wales 2006, Australia. t.tran@physics.usyd.edu.au
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MeSH Terms
Descriptor/Qualifier:
Biofilms / growth & development*
Cell Count
Immobilization / methods*
Models, Chemical*
Photoelectron Spectroscopy
Polytetrafluoroethylene / chemistry*
Saccharomyces cerevisiae / physiology*
Spectroscopy, Fourier Transform Infrared
Surface Properties
Chemical
Reg. No./Substance:
9002-84-0/Polytetrafluoroethylene
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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