| Ion channels underlying stimulus-exocytosis coupling and its cell-to-cell heterogeneity in beta-cells of transplantable porcine islets of Langerhans. | |
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MedLine Citation:
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PMID: 19242122 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Given the growing interest in porcine islets as model tissue for studying the pathogenesis of human diabetes mellitus and its treatment by transplantation, we investigated stimulus-exocytosis coupling in single porcine beta-cells using patch clamp electrophysiology, Ca2+ imaging, capacitance tracking and amperometry. We establish that porcine beta-cells display several features prominently seen in beta-cells from human islets of Langerhans. These include: (i) wide heterogeneity of electrical responsiveness to glucose; (ii) dependence of action potential activity on voltage-dependent Na(+) as well as high voltage activated Ca2+ current; (iii) heterogeneity of time course of depolarization-evoked insulin granule exocytosis; and (iv) the dependence of vigorous single cell electrical activity and insulin granule exocytosis on the presence of agents that enhance cytosolic cAMP concentration. These findings promote the usefulness of porcine beta-cells as a model for studying beta-cell function in large mammals, including humans, as well as an appropriate source of tissue for xenotransplantation. |
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Authors:
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Amelia M Silva; Adam S Dickey; David W Barnett; Stanley Misler |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2009-03-16 |
Journal Detail:
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Title: Channels (Austin, Tex.) Volume: 3 ISSN: 1933-6969 ISO Abbreviation: Channels (Austin) Publication Date: 2009 Mar-Apr |
Date Detail:
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Created Date: 2009-05-11 Completed Date: 2009-09-01 Revised Date: 2010-01-15 |
Medline Journal Info:
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Nlm Unique ID: 101321614 Medline TA: Channels (Austin) Country: United States |
Other Details:
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Languages: eng Pagination: 91-100 Citation Subset: IM |
Affiliation:
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Departments of Internal Medicine and Cell Biology/Physiology, Washington University Medical Center, Saint Louis, MO 63110, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Action Potentials Animals Calcium / metabolism Electrophysiology Exocytosis* Glucose / pharmacology Humans Insulin-Secreting Cells / physiology* Ion Channels / metabolism* Islets of Langerhans / cytology, physiology Islets of Langerhans Transplantation* Models, Animal Sodium / metabolism Swine |
| Grant Support | |
ID/Acronym/Agency:
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4R01-DK37380/DK/NIDDK NIH HHS; 5T32 GM07281/GM/NIGMS NIH HHS; R0I-DK20579/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Ion Channels; 50-99-7/Glucose; 7440-23-5/Sodium; 7440-70-2/Calcium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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