Document Detail


Ion channels in volume regulation of clonal kidney cells.
MedLine Citation:
PMID:  21039991     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVES: Clonal kidney cells (Vero cells) are extensively utilized in the manufacture of biological preparations for disease diagnostics and therapeutics and also in preparation of vaccines. In all cells, regulation of volume is an essential function coupled to a variety of physiological processes and is a topic of interest. The objective here was to investigate involvement of ion channels in the process of volume regulation of Vero cells.
METHODS: Involvement of ion channels in cell volume regulation was studied using video-microscopy and flow cytometry. Pharmacologically unaltered cells of different sizes, which are presumably at different phases of the cell cycle, were used.
RESULTS: Ion transport inhibitors altered all phases of regulatory volume decrease (RVD) of Vero cells, rate of initial cell swelling, V(max) and volume recovery. Effects were dependent on type of inhibitor and on cell size (cell cycle phase). Participation of aquaporins in RVD was suggested. Inhibitors decelerated growth, arresting Vero cells at the G(0) /G(1) phase boundary. Electrophysiological study confirmed presence of volume-activated Cl(-) channels and K(+) channels in plasmatic membranes of the cells.
CONCLUSION: Vero cells of all sizes maintained the ability to recover from osmotic swelling. Activity of ion channels was one of the key factors that controlled volume regulation and proliferation of the cells.
Authors:
M B da Silva; V M A Costa; V R A Pereira; G J B de Albertim; E B B de Melo; D P Bezerra; R P da Silva; C G Rodrigues; C M M Carneiro; L N Yuldasheva; O V Krasilnikov
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell proliferation     Volume:  43     ISSN:  1365-2184     ISO Abbreviation:  Cell Prolif.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-11-02     Completed Date:  2010-12-02     Revised Date:  2011-01-19    
Medline Journal Info:
Nlm Unique ID:  9105195     Medline TA:  Cell Prolif     Country:  England    
Other Details:
Languages:  eng     Pagination:  529-41     Citation Subset:  IM    
Copyright Information:
© 2010 Blackwell Publishing Ltd.
Affiliation:
Department of Biophysics and Radiobiology, Federal University of Pernambuco, Recife, PE, Brazil.
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MeSH Terms
Descriptor/Qualifier:
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / pharmacology
Animals
Cell Cycle / drug effects
Cell Proliferation / drug effects
Cell Size* / drug effects
Cells, Cultured
Cercopithecus aethiops
Flow Cytometry
Glyburide / pharmacology
Ion Channels / antagonists & inhibitors,  metabolism*
Kidney / cytology*,  metabolism*
Microscopy
Nitrobenzoates / pharmacology
Tetraethylammonium / pharmacology
Vero Cells
Chemical
Reg. No./Substance:
0/Ion Channels; 0/Nitrobenzoates; 10238-21-8/Glyburide; 107254-86-4/5-nitro-2-(3-phenylpropylamino)benzoic acid; 53005-05-3/4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; 66-40-0/Tetraethylammonium
Comments/Corrections
Erratum In:
Cell Prolif. 2011 Feb;44(1):109

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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