Document Detail


Ion mobility separation of variant histone tails extending to the "middle-down" range.
MedLine Citation:
PMID:  22559289     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Differential ion mobility spectrometry (FAIMS) can baseline-resolve multiple variants of post-translationally modified peptides extending to the 3-4 kDa range, which differ in the localization of a PTM as small as acetylation. Essentially orthogonal separations for different charge states expand the total peak capacity with the number of observed states that increases for longer polypeptides. This potentially enables resolving localization variants for yet larger peptides and even intact proteins.
Authors:
Alexandre A Shvartsburg; Yupeng Zheng; Richard D Smith; Neil L Kelleher
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-05-03
Journal Detail:
Title:  Analytical chemistry     Volume:  84     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-05-15     Completed Date:  2012-09-19     Revised Date:  2013-06-25    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4271-6     Citation Subset:  IM    
Affiliation:
Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99352, United States. alexandre.shvartsburg@pnl.gov
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MeSH Terms
Descriptor/Qualifier:
Acetylation
Helium / chemistry
Histones / analysis*,  isolation & purification,  metabolism
Hydrogen / chemistry
Ions / chemistry*
Mass Spectrometry*
Nitrogen / chemistry
Peptides / analysis
Grant Support
ID/Acronym/Agency:
CA 143869/CA/NCI NIH HHS; CA 155252/CA/NCI NIH HHS; GM 067193-09/GM/NIGMS NIH HHS; GM 103493-10/GM/NIGMS NIH HHS; P41 RR018522/RR/NCRR NIH HHS; R01 GM067193/GM/NIGMS NIH HHS; RR18522/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Histones; 0/Ions; 0/Peptides; 1333-74-0/Hydrogen; 7440-59-7/Helium; 7727-37-9/Nitrogen
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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