Document Detail

Iodixanol induces apoptotic and antiproliferative effects but no necrotic cell death in renal proximal tubular cells in vitro.
MedLine Citation:
PMID:  19291604     Owner:  NLM     Status:  MEDLINE    
PURPOSE: To evaluate the cytotoxic effects of the iso-osmolar contrast medium iodixanol on renal tubular cell cultures. MATERIALS AND METHODS: LLC-PK1 cells were incubated with iodixanol and isotonic NaCl (18.75 - 75 mg I/ml, 1 - 24 hours). Cell death was assessed by the trypan blue exclusion test. To assess apoptosis, mononucleosomes and oligonucleosomes of cell lysates were determined. Measurement of BrdU (5-bromo-2'-deoxyuridine) incorporation into the DNA was used for the quantification of cell proliferation. RESULTS: Iodixanol did not induce any significant increase in the number of necrotic cells (8 % and 9 % at 37.5 and 75 mg I/ml vs. 8 % for control, p > 0.05). In contrast, iodixanol significantly increased the number of oligonucleosomes indicating induction of apoptosis (125 +/- 4 % of control, p < 0.05). Iodixanol induced a significant, dose- and time-dependent inhibition of BrdU incorporation indicating the inhibition of cell proliferation (92 +/- 2 % and 79 +/- 2 % of control at 18.75 and 37.5 mg I/ml, p < 0.001). CONCLUSION: Apoptosis plus an antiproliferative effect of iodixanol without cell necrosis contribute to the renal tubular toxicity of iodixanol.
M C Heinrich; M Scheer; M Heckmann; M A Kuefner; M Uder
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Publication Detail:
Type:  Journal Article     Date:  2009-03-16
Journal Detail:
Title:  RöFo : Fortschritte auf dem Gebiete der Röntgenstrahlen und der Nuklearmedizin     Volume:  181     ISSN:  1438-9010     ISO Abbreviation:  Rofo     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-03-17     Completed Date:  2009-04-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7507497     Medline TA:  Rofo     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  349-54     Citation Subset:  IM    
Radiologisches Institut, Universitätsklinikum Erlangen.
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MeSH Terms
Apoptosis / drug effects*
Cell Line
Cell Proliferation / drug effects*
Contrast Media / toxicity*
Dose-Response Relationship, Drug
Kidney Tubules, Proximal / cytology*,  drug effects*
Necrosis / chemically induced
Triiodobenzoic Acids / toxicity*
Reg. No./Substance:
0/Contrast Media; 0/Triiodobenzoic Acids; 92339-11-2/iodixanol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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