Document Detail


Iodine and IFN-gamma synergistically enhance intercellular adhesion molecule 1 expression on NOD.H2h4 mouse thyrocytes.
MedLine Citation:
PMID:  15944276     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
NOD.H2(h4) mice spontaneously develop autoimmune lymphocytic thyroiditis that mimics human Hashimoto's thyroiditis, a disease where iodine, IFN-gamma, and adhesion molecules have all been implicated in the pathogenesis. To study how iodine and IFN-gamma modulate the expression of ICAM-1, we analyzed NOD.H2(h4) thyrocytes in baseline conditions (day 0) and at several time points following supplementation of iodine in the drinking water. On day 0, a small percentage ( approximately 10%) of thyrocytes constitutively expressed ICAM-1. The expression gradually increased to 13, 25, and 41% on days 7, 14 and 28, respectively, returning to baseline (9%) on day 35. The initial ICAM-1 kinetics was paralleled by thyroidal infiltration of CD45(+) hemopoietic cells, which increased from an average of 4% on day 0 to an average of 13, 21, and 24% on days 14, 28, and 35, respectively. To distinguish whether the observed ICAM-1 increase was a direct effect of iodine or a consequence of the immune infiltrate, we treated mouse primary thyrocyte cultures with 0.01 mM sodium iodine and showed a 3-fold increased ICAM-1 expression. To assess interaction between IFN-gamma and iodine, we analyzed CD45 and ICAM-1expression on thyrocytes from NOD.H2(h4) wild-type and NOD.H2(h4) thyr-IFN-gamma transgenic littermates. Strikingly, IFN-gamma interacted synergistically with iodine to enhance ICAM-1 expression on thyrocytes. These findings suggest that iodine and IFN-gamma cooperate to promote thyroidal expression of ICAM-1 in this mouse model of thyroiditis, highlighting the complex interplay present in the pathogenesis of Hashimoto's thyroiditis.
Authors:
Rajni B Sharma; Judy D Alegria; Monica V Talor; Noel R Rose; Patrizio Caturegli; C Lynne Burek
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  174     ISSN:  0022-1767     ISO Abbreviation:  J. Immunol.     Publication Date:  2005 Jun 
Date Detail:
Created Date:  2005-06-09     Completed Date:  2005-08-31     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7740-5     Citation Subset:  AIM; IM    
Affiliation:
Department of Pathology, School of Medicine, Johns Hopkins Medical Institutions, Baltimore, MD 21205, USA.
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MeSH Terms
Descriptor/Qualifier:
Adjuvants, Immunologic / administration & dosage,  metabolism,  pharmacology*
Administration, Oral
Animals
Cells, Cultured
Disease Models, Animal
Drug Synergism
Female
Housing, Animal
Intercellular Adhesion Molecule-1 / biosynthesis*
Interferon-gamma / genetics,  metabolism,  physiology*
Male
Mice
Mice, Inbred A
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Inbred NOD
Mice, Transgenic
Sodium Iodide / administration & dosage*,  antagonists & inhibitors,  metabolism
Species Specificity
Thyroid Gland / cytology,  drug effects,  immunology*,  metabolism*
Thyroiditis, Autoimmune / genetics,  immunology*,  therapy
Up-Regulation / drug effects,  genetics,  immunology*
Grant Support
ID/Acronym/Agency:
DK42174/DK/NIDDK NIH HHS; DK55670/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Adjuvants, Immunologic; 126547-89-5/Intercellular Adhesion Molecule-1; 7681-82-5/Sodium Iodide; 82115-62-6/Interferon-gamma

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