Document Detail


Involvement of two latex-clearing proteins during rubber degradation and insights into the subsequent degradation pathway revealed by the genome sequence of Gordonia polyisoprenivorans strain VH2.
MedLine Citation:
PMID:  22327575     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The increasing production of synthetic and natural poly(cis-1,4-isoprene) rubber leads to huge challenges in waste management. Only a few bacteria are known to degrade rubber, and little is known about the mechanism of microbial rubber degradation. The genome of Gordonia polyisoprenivorans strain VH2, which is one of the most effective rubber-degrading bacteria, was sequenced and annotated to elucidate the degradation pathway and other features of this actinomycete. The genome consists of a circular chromosome of 5,669,805 bp and a circular plasmid of 174,494 bp with average GC contents of 67.0% and 65.7%, respectively. It contains 5,110 putative protein-coding sequences, including many candidate genes responsible for rubber degradation and other biotechnically relevant pathways. Furthermore, we detected two homologues of a latex-clearing protein, which is supposed to be a key enzyme in rubber degradation. The deletion of these two genes for the first time revealed clear evidence that latex-clearing protein is essential for the microbial utilization of rubber. Based on the genome sequence, we predict a pathway for the microbial degradation of rubber which is supported by previous and current data on transposon mutagenesis, deletion mutants, applied comparative genomics, and literature search.
Authors:
Sebastian Hiessl; Jörg Schuldes; Andrea Thürmer; Tobias Halbsguth; Daniel Bröker; Angel Angelov; Wolfgang Liebl; Rolf Daniel; Alexander Steinbüchel
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-02-10
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  78     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-03-26     Completed Date:  2012-07-09     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2874-87     Citation Subset:  IM    
Affiliation:
Department of Veterinary Science and Microbiology, The University of Arizona, Tucson, Arizona, USA.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/CP003119;  CP003120
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MeSH Terms
Descriptor/Qualifier:
Actinomycetales / genetics*,  metabolism*
Bacterial Proteins / genetics*,  metabolism*
Base Composition
Chromosomes, Bacterial
DNA Transposable Elements
DNA, Bacterial / chemistry,  genetics
Gene Deletion
Genome, Bacterial
Latex / metabolism*
Metabolic Networks and Pathways / genetics*
Molecular Sequence Data
Mutagenesis, Insertional
Rubber / metabolism*
Sequence Analysis, DNA
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/DNA Transposable Elements; 0/DNA, Bacterial; 0/Latex; 9006-04-6/Rubber
Comments/Corrections

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