Document Detail

Involvement of triacylglycerol in the metabolism of fatty acids by cultured neuroblastoma and glioma cells.
MedLine Citation:
PMID:  7161559     Owner:  NLM     Status:  MEDLINE    
The metabolism (chain elongation, desaturation, and incorporation into complex lipids) of thirteen different radiolabeled fatty acids and acetate was examined in N1E-115 neuroblastoma and C-6 glioma cell lines in culture. During 6-hr incubations, all fatty acids were extensively (14-80%) esterified to complex lipids, mainly choline phosphoglycerides and triacylglycerol. With trienoic and tetraenoic substrates, inositol and ethanolamine phosphoglycerides also contained up to 30% of the labeled fatty acids; plasmalogen contained up to half of the label in the ethanolamine phosphoglyceride fraction of neuroblastoma cells. Chain elongation and delta 9, delta 6, and delta 5 desaturation occurred in both cell lines; delta 4 desaturation was not observed. Seemingly anomalous utilization of arachidic acid and some selectivity based on the geometric configuration of double bonds was observed. These studies indicate that these cell lines are capable of modulating cellular membrane composition by a combination of selective exclusion and removal of inappropriate acyl chains and of modification of other acyl chains by desaturation and chain elongation. The time courses and patterns of modification and incorporation of exogenous substrates into phospholipids and triacylglycerol suggest that exogenous unsaturated fatty acid may be incorporated into triacylglycerol and later released for further metabolism and incorporation into phospholipids. This supports a role for triacylglycerol in the synthesis of membrane complex lipids in cell lines derived from neural tissue.
H W Cook; J T Clarke; M W Spence
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of lipid research     Volume:  23     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  1982 Dec 
Date Detail:
Created Date:  1983-04-15     Completed Date:  1983-04-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1292-300     Citation Subset:  IM    
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MeSH Terms
Carbon Radioisotopes / diagnostic use
Cell Line
Fatty Acids / metabolism*
Fatty Acids, Nonesterified / metabolism
Glioma / metabolism*
Lipids / biosynthesis*
Neuroblastoma / metabolism*
Phospholipids / biosynthesis
Structure-Activity Relationship
Triglycerides / metabolism*
Reg. No./Substance:
0/Carbon Radioisotopes; 0/Fatty Acids; 0/Fatty Acids, Nonesterified; 0/Lipids; 0/Phospholipids; 0/Triglycerides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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