| Involvement of the sphingolipid ceramide in heat-shock-induced apoptosis of bovine oocytes. | |
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MedLine Citation:
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PMID: 21871207 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Programmed cell death via the sphingomyelin pathway has been suggested to underlie heat-shock disturbance of oocyte developmental competence. A series of experiments were performed to characterise the role of the sphingolipid ceramide in heat-shock-induced apoptosis, and to determine whether ceramide formation can be regulated. Bovine cumulus-oocyte complexes (COCs) were aspirated from ovaries collected in the cold season (November-April), in vitro-matured, fertilised and cultured for 8 days. Exposure of COCs to heat shock (41°C) during maturation reduced cleavage rate and blastocyst formation relative to the control group (38.5°C). Annexin-V binding (V-FITC assay), which is associated with the early apoptotic event of membrane phosphatidylserine turnover, was higher in oocytes exposed to short-term versus long-term heat shock, suggesting that heat-shock-induced apoptosis involves membrane alterations. Similar to heat exposure, oocyte maturation with C(2)-ceramide had a dose-dependent deleterious effect on the first cleavages and subsequent embryonic development in association with increased annexin-V binding. Blocking endogenous ceramide generation with fumonisin B1, a specific inhibitor of dihydroceramide synthase (i.e. de novo formation), moderated, to some extent, the effects of heat shock on oocyte developmental competence, suggesting that ceramide plays an important role in heat-shock-induced apoptosis. |
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Authors:
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Dorit Kalo; Zvi Roth |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Reproduction, fertility, and development Volume: 23 ISSN: 1031-3613 ISO Abbreviation: Reprod. Fertil. Dev. Publication Date: 2011 Sep |
Date Detail:
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Created Date: 2011-08-29 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8907465 Medline TA: Reprod Fertil Dev Country: Australia |
Other Details:
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Languages: eng Pagination: 876-88 Citation Subset: IM |
Affiliation:
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Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University, Rehovot 76100, Israel. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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