| Involvement of small GTPases Rho and Rac in the invasion of rat ascites hepatoma cells. | |
| | |
MedLine Citation:
|
PMID: 10411106 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Lysophosphatidic acid (LPA) triggers the invasion of a mesothelial cell monolayer by rat ascites hepatoma (MM1) cells. LPA also induces rapid morphological changes of MM1 cells, cell surface blebbing and pseudopodia formation. Pseudopodia formation is tightly correlated with cellular invasiveness. Clostridium Botulinum C3 exoenzyme and genistein abrogated the formation of blebs and pseudopodia together with the inhibition of invasion, indicating that GTPase Rho and certain tyrosine kinases are involved in both processes. MM1 cells expressing constitutively active Rho exhibited the invasion and the formation of blebs and pseudopodia in the absence of LPA. In contrast, MM1 cells expressing constitutively active Rac were not invasive in the absence of LPA, but were invasive in the presence of LPA. Their morphological response to LPA was almost the same as that of parental MM1 cells. Expression of dominant negative Rac suppressed the invasiveness to approximately 3% of that of parental MM1 cells, together with the inhibition of pseudopodia formation. Thus, Rho and Rac are cooperatively involved in both the invasion and the related morphological changes of MM1 cells. Rho activation is sufficient both for the induction of invasion and the morphological changes leading to the invasion, whereas Rac activation is necessary but not sufficient by itself. We propose that Rho activation is not mediated by Rac but the cooperation of both GTPases is essential to trigger the invasive behavior of MM1 cells. |
| | |
Authors:
|
F Imamura; M Mukai; M Ayaki; K Takemura; T Horai; K Shinkai; H Nakamura; H Akedo |
Related Documents
:
|
908456 - The spontaneous release of 51cr from labeled el-4 ascitic lymphoma cells as dependent u... 3795626 - Studies on responsiveness of hepatoma cells to catecholamines. iv. lack of adrenergic a... 8737236 - Characterization of gangliosides from ehrlich ascites tumour cells and their variants. 6200416 - The specificity of human liver membrane lipoprotein: studies with monoclonal antibodies. 8512086 - Fetal antigen 1 (fa1) in the human pancreas: cell type expression, topological and quan... 2513726 - Nbd-taurine uptake by alpha-type carbonic anhydrase cells of turtle bladder. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Clinical & experimental metastasis Volume: 17 ISSN: 0262-0898 ISO Abbreviation: Clin. Exp. Metastasis Publication Date: 1999 Mar |
Date Detail:
|
Created Date: 1999-08-05 Completed Date: 1999-08-05 Revised Date: 2009-11-19 |
Medline Journal Info:
|
Nlm Unique ID: 8409970 Medline TA: Clin Exp Metastasis Country: NETHERLANDS |
Other Details:
|
Languages: eng Pagination: 141-8 Citation Subset: IM |
Affiliation:
|
Department of Pulmonary Oncology, Osaka Medical Center for Cancer and Cardiovascular Diseases, Japan. fiman@a1.mbn.or.up |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Ascitic Fluid / enzymology, pathology* Blotting, Western Clostridium botulinum / enzymology Epithelium / enzymology GTP Phosphohydrolases / genetics, metabolism, physiology* GTPase-Activating Proteins Genistein / pharmacology Liver Neoplasms, Experimental / enzymology*, pathology* Lysophospholipids / pharmacology* Microscopy Neoplasm Invasiveness Protein-Tyrosine Kinases / physiology Proteins / physiology* Pseudopodia / pathology Rats Reverse Transcriptase Polymerase Chain Reaction Transfection Tumor Cells, Cultured rho GTP-Binding Proteins / metabolism, physiology* |
| Chemical | |
Reg. No./Substance:
|
0/GTPase-Activating Proteins; 0/Lysophospholipids; 0/Proteins; 446-72-0/Genistein; EC 2.7.10.1/Protein-Tyrosine Kinases; EC 3.6.1.-/GTP Phosphohydrolases; EC 3.6.5.2/rho GTP-Binding Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Matrix-degrading proteinases are shed in membrane vesicles by ovarian cancer cells in vivo and in vi...
Next Document: Murine hepatic microvascular adhesion molecule expression is inducible and has a zonal distribution.