Document Detail


Involvement of retinoic acid-induced transglutaminase activity in zonal differences of hepatocyte proliferation after partial hepatectomy.
MedLine Citation:
PMID:  16984597     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The authors have recently demonstrated that there is inverse correlation between transglutaminase (TGase) activity and DNA synthesis in periportal hepatocytes (PPH) and perivenous hepatocytes (PVH) at 1 day after partial hepatectomy in rats. In order to ensure the involvement of TGase in the differential growth capacities between periportal and perivenous regions of regenerating liver, the aim of this study was to investigate the effect of retinoic acid, an inducer of TGase expression, on zonal differences of hepatocyte proliferation between PPH and PVH isolated from regenerating rat liver. METHODS: Regenerating liver was prepared by 70% partial hepatectomy. PPH and PVH subpopulations were isolated by the digitonin/collagenase perfusion technique. Cell cycle was evaluated for incorporation of BrdU into hepatocytes and detected by flow cytometric analysis. TGase activity was determined by incorporation of 14C-putrescine into dimethylcasein. RESULTS: When retinoic acid was injected immediately after partial hepatectomy, TGase activity greatly increased in both PPH and PVH at 1 day after partial hepatectomy, and activity was higher in PPH than in PVH. DNA synthesis in both subpopulations did not increase 1 day after partial hepatectomy, with peaks of DNA synthesis shifting to 2 days, and synthesis was higher in PVH than in PPH. CONCLUSION: These results suggest that TGase might be involved in differential growth capacities between periportal and perivenous regions of regenerating rat liver after partial hepatectomy.
Authors:
Yosuke Ohtake; Akiko Maruko; Shinya Abe; Tomomi Nagashima; Manabu Fukumoto; Yasuhito Ohkubo
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of gastroenterology and hepatology     Volume:  21     ISSN:  0815-9319     ISO Abbreviation:  J. Gastroenterol. Hepatol.     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-09-20     Completed Date:  2007-01-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8607909     Medline TA:  J Gastroenterol Hepatol     Country:  Australia    
Other Details:
Languages:  eng     Pagination:  1726-30     Citation Subset:  IM    
Affiliation:
Department of Radiopharmacy, Tohoku Pharmaceutical University, Sendai, Miyagi, Japan. y-ohtake@tokoku-pharm.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Division
DNA Replication
Hepatectomy / methods*
Hepatocytes / enzymology*,  metabolism
Injections, Intraperitoneal
Liver / blood supply,  enzymology*
Liver Regeneration / physiology*
Male
Rats
Rats, Wistar
Statistics, Nonparametric
Transglutaminases / metabolism*
Tretinoin / administration & dosage,  pharmacology*
Chemical
Reg. No./Substance:
302-79-4/Tretinoin; EC 2.3.2.13/Transglutaminases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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