Document Detail


Involvement of reactive oxygen species generated from melanin synthesis pathway in phytotoxicty of L-DOPA.
MedLine Citation:
PMID:  15856781     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
L-DOPA is an active allelochemical that inhibits plant growth. To determine whether the phytotoxicity is due to the reactive oxygen species generated during its oxidation to melanin, oxidative damage, melanin accumulation, and the effect of antioxidants on its phytotoxicity were examined in L-DOPA-tolerant (barnyard grass) and -susceptible (lettuce) plants, and in suspension-cultured carrot cells. L-DOPA suppressed root elongation in lettuce compared to barnyard grass. Levels of melanin and thiobarbituric acid reactive substances (TBARS) increased remarkably in L-DOPA-treated lettuce roots, but not in barnyard grass. L-DOPA also suppressed carrot cell growth to 60% of the control at 1 mM. Melanin content in 1 mM L-DOPA-treated carrot cells increased continuously; however, ascorbic acid and alpha-tocopherol suppressed accumulation. When melanin formation was inhibited by ascorbic acid and alpha-tocopherol, growth of L-DOPA-treated cells was restored. TBARS levels were higher in 1 mM L-DOPA-treated carrot cells than in untreated control cells 2 d after treatment, but not at 4 or 6 d. Ascorbic acid and alpha-tocopherol suppressed the production of lipid peroxide during the initial 2 d. These results suggest that the phytotoxicity of L-DOPA is due to oxidative stress caused by reactive oxygen species from the melanin synthesis pathway.
Authors:
Mayumi Hachinohe; Hiroshi Matsumoto
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of chemical ecology     Volume:  31     ISSN:  0098-0331     ISO Abbreviation:  J. Chem. Ecol.     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2005-04-28     Completed Date:  2005-08-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7505563     Medline TA:  J Chem Ecol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  237-46     Citation Subset:  IM    
Affiliation:
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, lbaraki 305-8572, Japan.
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MeSH Terms
Descriptor/Qualifier:
Antioxidants / pharmacology*
Ascorbic Acid / pharmacology
Daucus carota / cytology,  metabolism
Dopamine / toxicity
Lettuce / cytology,  metabolism
Levodopa / toxicity*
Lipid Peroxidation / physiology
Melanins / analysis,  metabolism*
Oxidative Stress / drug effects*
Plant Roots / chemistry
Poaceae / cytology,  metabolism
Reactive Oxygen Species / metabolism*
Thiobarbituric Acid Reactive Substances / analysis,  metabolism
Time Factors
alpha-Tocopherol / pharmacology
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Levodopa; 0/Melanins; 0/Reactive Oxygen Species; 0/Thiobarbituric Acid Reactive Substances; 50-81-7/Ascorbic Acid; 59-02-9/alpha-Tocopherol

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