Document Detail

Involvement of protein kinase Cepsilon in the stimulation of anionic amino acid transport in cultured human fibroblasts.
MedLine Citation:
PMID:  8824256     Owner:  NLM     Status:  MEDLINE    
Protein kinase C (PKC) activation stimulates transport system X-AG for anionic amino acids in cultured human fibroblasts (Franchi-Gazzola, R., Visigalli, R., Bussolati, O., and Gazzola, G. C. (1994) FEBS Lett. 352, 109-112). To identify which PKC isoform is responsible for this effect, aspartate transport through system X-AG, PKC activity, and the subcellular distribution of PKC isoforms have been studied before and after treatment with phorbol 12, 13-dibutyrate (PDBu) in fibroblasts maintained at low serum for 1 (control cells) or 7 days (quiescent cells). In control cells aspartate transport and PKC activity in the particulate fraction were stimulated by short term PDBu treatment; both stimulatory effects were down-regulated by a prolonged exposure to the phorbol. In contrast, in quiescent cells aspartate transport and particulate PKC activity were higher than control under basal conditions, unaffected by a short term PDBu treatment, and lowered by a prolonged incubation with the phorbol. In both control and quiescent cells a short term PDBu treatment modified PKCalpha distribution, increasing its membrane-associated fraction. PKCdelta was mostly in the soluble fraction and scarcely sensitive to PDBu. A brief exposure to PDBu increased membrane-associated PKCepsilon in control but not in quiescent cells. In these cells epsilon isoform was found exclusively in the particulate fraction even in PDBu-untreated cells. A prolonged PDBu treatment caused a partial down-regulation of membrane-associated PKCepsilon in control cells and its marked decrease in quiescent cells. It is concluded that PKC-dependent changes in system X-AG activity parallel the behavior of PKCepsilon, thus suggesting a specific role for this isoform in system X-AG regulation.
R Franchi-Gazzola; R Visigalli; O Bussolati; G C Gazzola
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  271     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1996 Oct 
Date Detail:
Created Date:  1996-11-26     Completed Date:  1996-11-26     Revised Date:  2012-06-25    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  26124-30     Citation Subset:  IM    
Istituto di Patologia Generale, Università degli Studi di Parma, I-43100 Parma, Italy.
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MeSH Terms
Amino Acid Transport Systems
Amino Acids / metabolism*
Biological Transport
Blotting, Western
Carrier Proteins / metabolism*
Cells, Cultured
Down-Regulation / drug effects
Fibroblasts / enzymology
Isoenzymes / metabolism*
Microscopy, Confocal
Phorbol 12,13-Dibutyrate / metabolism
Protein Kinase C / metabolism*
Protein Kinase C-epsilon
Reg. No./Substance:
0/Amino Acid Transport Systems; 0/Amino Acids; 0/Carrier Proteins; 0/Isoenzymes; 37558-16-0/Phorbol 12,13-Dibutyrate; EC protein, human; EC Kinase C; EC Kinase C-epsilon

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