| Involvement of protein kinase C and E2F-5 in euxanthone-induced neurite differentiation of neuroblastoma. | |
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MedLine Citation:
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PMID: 16546434 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Euxanthone, a neuritogenic agent isolated from the medicinal herb Polygala caudata, has been shown to induce morphological differentiation and neurite outgrowth in murine neuroblastoma Neuro 2a cells (BU-1 subclone). In order to elucidate the underlying mechanisms of euxanthone-induced neurite outgrowth, a proteomic approach was employed. In the present study, two dimensional (2-D) gel electrophoresis and matrix-assisted laser desorption/ionization-time of flight (MALDI-ToF) mass spectrometry were performed to investigate the alterations in protein expression profile of euxanthone-treated BU-1 cells. Fourteen identified proteins were changed in expression levels after induction of neurite growth. These proteins included participants in transcription and cell cycle regulation, calcium influx and calcium signaling, fatty acid metabolism, cytoskeleton reorganization, casein kinase signal transduction, putative transbilayer amphipath transport and protein biosynthesis. Among the 14 identified proteins, E2F transcription factor 5 (E2F-5) was significantly up-regulated after euxanthone treatment. Go6976, a protein kinase C (PKC) alpha/betaI inhibitor, was found to inhibit neuritogenesis and expression of E2F-5 in the euxanthone-treated BU-1 cells, while SH-6, the Akt/PKB inhibitor, had no inhibitory effect. The gene silencing of E2F-5 by small interfering RNA (siRNA) was found to abolish the euxanthone-induced neurite outgrowth. In conclusion, these results indicated that the transcription factor E2F-5 was actively involved in the regulation of euxanthone-induced neurite outgrowth via PKC pathway. |
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Authors:
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Wai Yan Ha; Pui Kei Wu; Tsz Wai Kok; Kar Wah Leung; Nai Ki Mak; Patrick Ying Kit Yue; Sai Ming Ngai; Sau Na Tsai; Ricky Ngok Shun Wong |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-03-02 |
Journal Detail:
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Title: The international journal of biochemistry & cell biology Volume: 38 ISSN: 1357-2725 ISO Abbreviation: Int. J. Biochem. Cell Biol. Publication Date: 2006 |
Date Detail:
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Created Date: 2006-05-08 Completed Date: 2006-09-05 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 9508482 Medline TA: Int J Biochem Cell Biol Country: England |
Other Details:
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Languages: eng Pagination: 1393-401 Citation Subset: IM |
Affiliation:
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Research and Development Division, School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong SAR, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carbazoles / pharmacology Cell Differentiation / drug effects* Cell Line, Tumor E2F Transcription Factors / genetics, metabolism* Electrophoresis, Gel, Two-Dimensional Gene Silencing Indoles / pharmacology Neurites / drug effects, metabolism* Neuroblastoma / metabolism, pathology Protein Kinase C / antagonists & inhibitors, metabolism* Protein Kinase Inhibitors / pharmacology Proteomics / methods Proto-Oncogene Proteins c-akt / antagonists & inhibitors, metabolism Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Xanthones / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Carbazoles; 0/E2F Transcription Factors; 0/Indoles; 0/Protein Kinase Inhibitors; 0/Xanthones; 136194-77-9/Go 6976; 529-61-3/euxanthone; EC 2.7.11.1/Proto-Oncogene Proteins c-akt; EC 2.7.11.13/Protein Kinase C |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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