Document Detail

Involvement of pro-apoptotic and anti-apoptotic factors in the early development of the human pituitary gland.
MedLine Citation:
PMID:  18712678     Owner:  NLM     Status:  MEDLINE    
The spatial and temporal pattern of appearance of pro-apoptotic caspase-3 and p53 proteins, and anti-apoptotic bcl-2 protein was investigated in the developing pituitary gland of 6 human embryos 5-8-weeks old, using morphological and immunohistochemical techniques. Their dynamic appearance was analyzed in the Rathke's pouch (future adenohypophysis), mesenchyme, and in the developing neurohypophysis. In the 5th and 6th week, caspase-3 positive cells appeared in the Rathke's pouch (5%) and stalk (11%), in the mesenchyme, but not in the neurohypophysis. In the 6th and 7th week, apoptotic cells were more numerous in the caudal part of the Rathke's pouch due to its separation from the oral epithelium. Pro-apoptotic p53 protein was detected in all parts of the pituitary gland throughout the investigated period. Nuclear condensations characterized cells positive to caspase-3 and p53 proteins. Apoptotic cells displayed condensations of nuclear chromatin on an ultrastructural level as well. While caspase-3 dependent pathway of cell death participated in morphogenesis of the adenohypophysis and associated connective tissue, p53-mediated apoptosis most likely participates in morphogenesis of all parts of the gland, including neurohypophysis. The anti-apoptotic bcl-2 protein was also detected in all parts of the developing gland. With advancing development, the positivity to bcl-2 protein increased in the cells of the adenohypophysis, while it decreased in the neurohypophysis. Bcl-2 protein probably prevented cell death in all parts of the gland and enhanced cell differentiation. The described pattern of appearance of the investigated pro-apoptotic and anti-apoptotic factors might be important for normal morphogenesis and function of the pituitary gland.
M Saraga-Babic; M Bazina; K Vukojevic; I Bocina; V Stefanovic
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Histology and histopathology     Volume:  23     ISSN:  1699-5848     ISO Abbreviation:  Histol. Histopathol.     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-08-20     Completed Date:  2008-10-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8609357     Medline TA:  Histol Histopathol     Country:  Spain    
Other Details:
Languages:  eng     Pagination:  1259-68     Citation Subset:  IM    
Department of Anatomy, Histology and Embryology, School of Medicine, University of Split, Split, Croatia.
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MeSH Terms
Apoptosis Regulatory Proteins / metabolism*
Caspase 3 / metabolism
Cell Differentiation
Gestational Age
In Situ Nick-End Labeling
Pituitary Gland / embryology,  enzymology,  metabolism*
Pituitary Gland, Posterior / embryology,  metabolism
Proto-Oncogene Proteins c-bcl-2 / metabolism
Tumor Suppressor Protein p53 / metabolism
Reg. No./Substance:
0/Apoptosis Regulatory Proteins; 0/Proto-Oncogene Proteins c-bcl-2; 0/TP53 protein, human; 0/Tumor Suppressor Protein p53; EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/Caspase 3

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