| Involvement of polyubiquitin chains via specific chain linkages in stress response in mammalian cells. | |
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MedLine Citation:
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PMID: 16327172 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Polyubiquitination plays key roles in various proteasome-dependent and independent cellular events. To elucidate roles in stress response of polyubiquitin chains formed via specific chain linkages in mammalian cells, we established NIH3T3 stable cell lines that are capable of conditionally expressing K29R, K48R and K63R ubiquitin mutants, in which the Lys29, Lys48 and Lys63 residues of ubiquitin had been changed to Arg, and we examined the effects of various stresses on their cell viabilities. The expression of K63R ubiquitin mutant decreased viability of the cells post-exposed to ethanol, H(2)O(2) and methyl methanesulfonate (MMS), while that of K48R mutant decreased viability of the cells post-exposed to heat shock as well as ethanol, H(2)O(2) and MMS. Thus, these results suggest that polyubiquitin chains formed via specific chain linkages are involved in the respective stress responses in mammalian cells. |
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Authors:
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Masahiro Fujimuro; Tadashi Nishiya; Yasuyuki Nomura; Hideyoshi Yokosawa |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biological & pharmaceutical bulletin Volume: 28 ISSN: 0918-6158 ISO Abbreviation: Biol. Pharm. Bull. Publication Date: 2005 Dec |
Date Detail:
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Created Date: 2005-12-05 Completed Date: 2006-03-09 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9311984 Medline TA: Biol Pharm Bull Country: Japan |
Other Details:
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Languages: eng Pagination: 2315-8 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arginine Blotting, Western Cell Shape / drug effects Cell Survival / drug effects DNA Damage / drug effects Ethanol / pharmacology Gene Expression / drug effects Heat Stress Disorders Heat-Shock Response / physiology* Hydrogen Peroxide / pharmacology Isopropyl Thiogalactoside / pharmacology Lysine Methyl Methanesulfonate / pharmacology Mice Molecular Conformation Mutation / drug effects NIH 3T3 Cells* Polyubiquitin / chemistry, genetics, pharmacology* Reverse Transcriptase Polymerase Chain Reaction |
| Chemical | |
Reg. No./Substance:
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120904-94-1/Polyubiquitin; 367-93-1/Isopropyl Thiogalactoside; 56-87-1/Lysine; 64-17-5/Ethanol; 66-27-3/Methyl Methanesulfonate; 74-79-3/Arginine; 7722-84-1/Hydrogen Peroxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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