| Involvement of polyphosphorylserine blocks in the Fe(III) binding by phosvitin. | |
| | |
MedLine Citation:
|
PMID: 975400 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
It has been suggested that the binding of iron(III) by phosvitin involves the phosphoric radicals of phosphorylserine residues, many of which are arranged in rows of several consecutive phosphoamino acids. In this paper we present evident that, unlike free phosphorylserine which does not interact with Fe(III), polyphosphorylserine blocks--(Ser-P)n, with n greater than or equal to 4 -- bind Fe(III) like phosvitin, though less actively, to give complexes stable at very acidic pHs. The binding of iron does not cause any polymerization of the phosphopeptides, and the (Ser-P)n-Fe(III) complexes display an Fe/P ratio significantly lower than 0.5, found in Fe-saturated phosvitin. These findings indicate that polyphosphorylserine blocks play an important role in the binding of iron by phosvitin, and that in the intact protein their binding capacity is optimized by the conformation of the polypeptide chain. |
| | |
Authors:
|
A Donella; L A Pinna; V Moret |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Chemico-biological interactions Volume: 15 ISSN: 0009-2797 ISO Abbreviation: Chem. Biol. Interact. Publication Date: 1976 Oct |
Date Detail:
|
Created Date: 1977-01-03 Completed Date: 1977-01-03 Revised Date: 2000-12-18 |
Medline Journal Info:
|
Nlm Unique ID: 0227276 Medline TA: Chem Biol Interact Country: NETHERLANDS |
Other Details:
|
Languages: eng Pagination: 165-71 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Binding Sites Egg Proteins* Iron* Kinetics Molecular Weight Organophosphorus Compounds Oxidation-Reduction Phosvitin* Serine* |
| Chemical | |
Reg. No./Substance:
|
0/Egg Proteins; 0/Organophosphorus Compounds; 56-45-1/Serine; 7439-89-6/Iron; 9008-96-2/Phosvitin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Identification of the persistently bound form of the carcinogen N-acetyl-2-aminofluorene to rat live...
Next Document: The distribution of [3H]synthanecine A bis-N-ethylcarbamate and its metabolites in the rat.