Document Detail

Involvement of poly(ADP-ribose) polymerase and activation of caspase-3-like protease in heat shock-induced apoptosis in tobacco suspension cells.
MedLine Citation:
PMID:  10828442     Owner:  NLM     Status:  MEDLINE    
The cleavage of poly(ADP-ribose) polymerase (PARP) by caspase (casp)-3 is an essential link in the apoptotic pathway in animal cells. In plant cells, however, there is no authentic evidence for the similar role that PARP may play during apoptosis. Using a heat shock (HS)-induced apoptosis system of tobacco cells, we found that immediately after a 4 h heat treatment, PARP was cleaved to form an 89 kDa signature fragment, while DNA laddering appeared only after a 20 h recovery following the HS. An activation of casp-3-like protease was also observed. The results suggest that apoptosis in plants and animals may share common mechanisms. On the other hand, when cells were preincubated with 4 mM 3-aminobenzamide or 2-8 mM nicotinamide, the specific inhibitors of PARP, before HS treatment, apoptotic cell death was reduced significantly. Our results thus imply that PARP may also be involved in apoptosis in a different way from the casp-related events.
R h Tian; G Y Zhang; C H Yan; Y R Dai
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  FEBS letters     Volume:  474     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  2000 May 
Date Detail:
Created Date:  2000-07-06     Completed Date:  2000-07-06     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  11-5     Citation Subset:  IM    
Department of Biology, Tsinghua University, 100084, Beijing, China.
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MeSH Terms
Apoptosis* / drug effects
Benzamides / pharmacology
Blotting, Western
Caspase 3
Caspases / metabolism*
Cells, Cultured
DNA Fragmentation
Enzyme Activation
Enzyme Inhibitors / pharmacology
Hot Temperature*
Niacinamide / pharmacology
Peptide Fragments / metabolism
Plants, Toxic*
Poly(ADP-ribose) Polymerases / antagonists & inhibitors,  metabolism*
Tobacco / cytology*
Reg. No./Substance:
0/Benzamides; 0/Enzyme Inhibitors; 0/Peptide Fragments; 3544-24-9/3-aminobenzamide; 98-92-0/Niacinamide; EC Polymerases; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases

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