Document Detail


Involvement of phospholipids in resistance and adaptation of Escherichia coli to acid conditions and to long-term survival.
MedLine Citation:
PMID:  12951243     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In Escherichia coli membranes, three major phospholipids are formed: phosphatidylethanolamine (PE), phosphatidylglycerol (PG) and cardiolipin (CL). We report here the survival of mutants lacking either PE or both PG and CL at an acid pHo and during long-term survival experiments. Stationary phase cultures of E. coli lacking PE are much more sensitive to acid shock (pHo 3) than the wild-type strain. Moreover, in the strain lacking PE, long-term survival in stationary phase is impaired and after 5 days no viable cells are recovered. The survival of an exponential phase culture to acid shock is known to be increased if the culture is exposed to moderately acid conditions (pHo 5) prior to a shift to pHo 3. If either PE or both PG and CL are missing, the exposure to pHo 5 does not increase the survival at pHo 3.
Authors:
Sylvain Canet; Martine Heyde; Raymond Portalier; Patrick Laloi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEMS microbiology letters     Volume:  225     ISSN:  0378-1097     ISO Abbreviation:  FEMS Microbiol. Lett.     Publication Date:  2003 Aug 
Date Detail:
Created Date:  2003-09-02     Completed Date:  2003-12-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7705721     Medline TA:  FEMS Microbiol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  207-11     Citation Subset:  IM    
Affiliation:
Unité de Microbiologie et Génétique, UMR CNRS 5122, Bât A. Lwoff, Université Lyon 1, F-69622 Villeurbanne Cedex, France.
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MeSH Terms
Descriptor/Qualifier:
Adaptation, Physiological*
CDPdiacylglycerol-Serine O-Phosphatidyltransferase / genetics,  metabolism
Cardiolipins / physiology
Cell Membrane / chemistry
Colony Count, Microbial
Escherichia coli / genetics,  growth & development,  metabolism,  physiology*
Hydrogen-Ion Concentration
Mutagenesis, Insertional
Phosphatidylethanolamines / physiology
Phosphatidylglycerols / physiology
Phospholipids / physiology*
Transferases (Other Substituted Phosphate Groups) / genetics,  metabolism
Chemical
Reg. No./Substance:
0/Cardiolipins; 0/Phosphatidylethanolamines; 0/Phosphatidylglycerols; 0/Phospholipids; EC 2.7.8.-/Transferases (Other Substituted Phosphate Groups); EC 2.7.8.5/CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase; EC 2.7.8.8/CDPdiacylglycerol-Serine O-Phosphatidyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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