Document Detail

Involvement of phosphatidylserine externalization in the down-regulation of c-myb expression in differentiating C2C12 cells.
MedLine Citation:
PMID:  17924964     Owner:  NLM     Status:  MEDLINE    
Analysis of c-myb gene down-regulation in differentiating C212 cells revealed that in proliferating cells, c-myb expression is high and ceases as the proliferation rate decreases. However, a low level of c-myb mRNA was detected in confluent non-proliferating differentiating cells for an extended period of time before it declined to an undetectable level. The time course of c-myb gene silencing in differentiating cells correlated with exposition of phosphatidylserine (PS) on the cell surface. Moreover, the interaction of exposed PS with exogenously added annexin V perturbed PS-mediated cell signaling and transiently up-regulated the declining c-myb expression. We, therefore, suggest that cell surface-exposed PS, which plays a role in the process of myotube formation, is also involved in the down-regulation of c-myb expression.
Petr Kaspar; Michal Dvorák
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-10-09
Journal Detail:
Title:  Differentiation; research in biological diversity     Volume:  76     ISSN:  1432-0436     ISO Abbreviation:  Differentiation     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-02-29     Completed Date:  2008-08-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0401650     Medline TA:  Differentiation     Country:  England    
Other Details:
Languages:  eng     Pagination:  245-52     Citation Subset:  IM    
Institute of Molecular Genetics, AS CR v.v.i., Vídenská 1083, Prague 4, CZ-14220, Czech Republic.
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MeSH Terms
Annexin A5 / metabolism
Base Sequence
Cell Differentiation*
Cell Line
DNA Primers
Fluorescent Antibody Technique
Phosphatidylserines / metabolism*
Proto-Oncogene Proteins c-myb / genetics*
RNA, Messenger / genetics
Reverse Transcriptase Polymerase Chain Reaction
Reg. No./Substance:
0/Annexin A5; 0/DNA Primers; 0/Phosphatidylserines; 0/Proto-Oncogene Proteins c-myb; 0/RNA, Messenger

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