Document Detail


Involvement of oxygen in harvesting injury of the liver. An experimental study including substrate free organ persufflation to evaluate a specific therapeutic approach.
MedLine Citation:
PMID:  1925067     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The present study was undertaken to assess the role of oxygen free radicals relating to cell damage upon reoxygenation of the ischemically altered isolated rat liver. Livers were excised and flushed via the portal vein with Ringer's solution and Euro-Collins solution, to which superoxide dismutase (SOD) was added in the experimental group. After warm ischemia at 37 degrees C and cold storage at 4 degrees C, the livers were reperfused via the portal vein with carbogen-saturated Krebs-Henseleit solution. Other livers were subjected to a retrograde persufflation via the infrahepatic caval vein with either oxygen or nitrogen and then rinsed with Ringer's solution. During reperfusion, SOD-treated livers showed markedly reduced vascular resistance, lower enzyme release and enhanced VO2 accordingly, energy charge at the end of reperfusion was significantly higher in the treated group. With reference to the tissue content of malondialdehyde, SOD-treated livers showed significantly less damage than the corroboration for these data. Enzyme activities in the eluate were significantly reduced under anoxic conditions as well as in the presence of SOD. We conclude from these data that oxygen free radicals do exert a detrimental impact on the reoxygenated liver, which could be specifically suppressed by application of exogenous SOD.
Authors:
T Minor; W Isselhard; G Kunz; S Saad
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Research in experimental medicine. Zeitschrift für die gesamte experimentelle Medizin einschliesslich experimenteller Chirurgie     Volume:  191     ISSN:  0300-9130     ISO Abbreviation:  Res Exp Med (Berl)     Publication Date:  1991  
Date Detail:
Created Date:  1991-10-28     Completed Date:  1991-10-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0324736     Medline TA:  Res Exp Med (Berl)     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  167-75     Citation Subset:  IM    
Affiliation:
Institut for Experimental Medicine, University of Cologne, Federal Republic of Germany.
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / metabolism
Animals
Energy Metabolism
Free Radicals
Lipid Peroxidation / drug effects
Liver / drug effects,  injuries*,  metabolism
Male
Oxygen / metabolism*
Rats
Rats, Inbred Strains
Reperfusion Injury / etiology*,  metabolism,  prevention & control
Superoxide Dismutase / pharmacology
Chemical
Reg. No./Substance:
0/Free Radicals; 56-65-5/Adenosine Triphosphate; 7782-44-7/Oxygen; EC 1.15.1.1/Superoxide Dismutase

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