Document Detail

Involvement of nitric oxide in the vasodilatory response to parathyroid hormone-related peptide in the isolated rabbit kidney.
MedLine Citation:
PMID:  8156733     Owner:  NLM     Status:  MEDLINE    
1. The present study was designed to explore the role of NO derived from L-arginine in the vasodilatory response to synthetic human parathyroid hormone-related peptide-(1-34) in the isolated rabbit kidney perfused in the presence of indomethacin (10 mumol/l) and preconstricted with noradrenaline (7.2 nmol/min). 2. Under control conditions, bolus administrations of acetylcholine (10 mumol/l), an NO-dependent renal vasodilator, verapamil (0.1 mmol/l), an NO-independent renal vasodilator, and parathyroid hormone-related peptide (87 nmol/l) decreased the preconstriction pressure, by 31%, 71% and 43%, respectively. 3. Bolus administration of 100 mumol/l NG-nitro-L-arginine-methyl ester caused a 20% increment in the perfusion pressure of the noradrenaline-preconstricted kidney. NG-nitro-L-arginine methyl ester inhibited the vasodilatory effect of acetylcholine and parathyroid hormone-related peptide, by 68% and 44%, respectively, but did not alter the verapamil-induced vasodilatation. 4. Unlike L-arginine, the bolus administration of 1 mumol/l of a mono-substituted L-arginine derivative, N-alpha-benzoyl-L-arginine ethyl ester, durable decreased the noradrenaline/NG-nitro-L-arginine methyl ester-induced preconstriction by 57%. 5. Both L-arginine and N-alpha-benzoyl-L-arginine ethyl ester effectively reversed the inhibition induced by NG-nitro-L-arginine methyl ester on the vasodilatation elicited by acetylcholine and parathyroid hormone-related peptide. 6. In conclusion, the formation of NO from L-arginine contributes a substantial part to the vasodilatory action of parathyroid hormone-related peptide. Therefore, parathyroid hormone-related peptide appears to have a place among the renal haemodynamically active substances, whose vasodilatory actions are tuned by NO.
U Simeoni; T Massfelder; C Saussine; C Judes; J Geisert; J J Helwig
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical science (London, England : 1979)     Volume:  86     ISSN:  0143-5221     ISO Abbreviation:  Clin. Sci.     Publication Date:  1994 Mar 
Date Detail:
Created Date:  1994-05-19     Completed Date:  1994-05-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7905731     Medline TA:  Clin Sci (Lond)     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  245-9     Citation Subset:  IM    
Laboratoire de Physiologie Cellulaire Rénale, Université Louis Pasteur (DRED AL67u20), Strasbourg, France.
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MeSH Terms
Acetylcholine / pharmacology
Arginine / analogs & derivatives*,  pharmacology
Kidney / drug effects
NG-Nitroarginine Methyl Ester
Nitric Oxide / metabolism*
Organ Culture Techniques
Parathyroid Hormone / pharmacology*
Parathyroid Hormone-Related Protein
Proteins / pharmacology*
Vasodilation / drug effects,  physiology*
Verapamil / pharmacology
Reg. No./Substance:
0/Parathyroid Hormone; 0/Parathyroid Hormone-Related Protein; 0/Proteins; 10102-43-9/Nitric Oxide; 50903-99-6/NG-Nitroarginine Methyl Ester; 51-84-3/Acetylcholine; 52-53-9/Verapamil; 74-79-3/Arginine

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