Document Detail


Involvement of nicotinamide adenine dinucleotide in the action of cholera toxin in vitro.
MedLine Citation:
PMID:  166378     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
NAD is a necessary cofactor for the activation of adenylate cyclase (ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1) by cholera toxin. Lysates of certain types of cell that hydrolyze their endogenous store of NAD after cell disruption respond poorly or not at all to cholera toxin. Lysates of pigeon erythrocytes, which lack enzymes that degrade NAD, provide a convenient and reproducible system for assaying the activity of cholera toxin in vitro and allow investigation of the mechanism of action of the toxin upon broken cells.
Authors:
D M Gill
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  72     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1975 Jun 
Date Detail:
Created Date:  1975-09-09     Completed Date:  1975-09-09     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2064-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenylate Cyclase / metabolism
Animals
Columbidae
Enzyme Activation
Erythrocytes / drug effects*,  enzymology,  metabolism
Hydrolysis
N-Glycosyl Hydrolases / pharmacology
NAD / analogs & derivatives,  metabolism,  pharmacology*
Neurospora / enzymology
Rabbits
Species Specificity
Structure-Activity Relationship
Toxins, Biological / pharmacology*
Turkeys
Vibrio cholerae*
Chemical
Reg. No./Substance:
0/Toxins, Biological; 53-84-9/NAD; EC 3.2.2.-/N-Glycosyl Hydrolases; EC 4.6.1.1/Adenylate Cyclase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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