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Involvement of neuronal NO synthase in collateral artery growth.
MedLine Citation:
PMID:  22053696     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
To evaluate the role of neuronal nitric oxides synthase (nNOS) in collateral artery growth (arteriogenesis), we analyzed the expression pattern of nNOS at distinct time points on RNA and protein levels in a rabbit and a murine model of peripheral arteriogenesis. In the rabbit model, Northern blot analyses revealed a significant upregulation of nNOS at 6 h (1.6-fold), 12 h (2.2-fold) and 24 h (2.0-fold) after induction of arteriogenesis via femoral artery ligation, when compared to the sham operated side. In mice, an upregulation of nNOS was also detected using Northern blot (at 6 h, 12 h) and qRT-PCR (12 h: 2.4-fold). On the protein level, nNOS was found to be upregulated 24 h after femoral artery ligation. Immunohistochemical staining showed that nNOS was localized in endothelial and smooth muscle cells of collateral arteries, as well as in skeletal muscle and nerves. In summary, our data provide evidence that nNOS is not constitutively expressed, but is induced during arteriogenesis, playing a role in supplying reactive oxygen species such as H2O2 and low levels of NO.
Authors:
J-I Pagel; J Borgolte; I Hoefer; B Fernández; W Schaper; E Deindl
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Indian journal of biochemistry & biophysics     Volume:  48     ISSN:  0301-1208     ISO Abbreviation:  Indian J. Biochem. Biophys.     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-11-07     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0310774     Medline TA:  Indian J Biochem Biophys     Country:  India    
Other Details:
Languages:  eng     Pagination:  270-4     Citation Subset:  IM    
Affiliation:
Walter-Brendel-Centre of Experimental Medicine, Ludwig-Maximilians-University Munich, Germany.
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