Document Detail


Involvement of midkine expression in the inhibitory effects of low-frequency magnetic fields on cancer cells.
MedLine Citation:
PMID:  21360556     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Effects of magnetic fields (MFs) on cancer cells may depend on cell type and exposure conditions. Gene expression levels are different among cancer cells. However, the effect of MFs on cancer cells with different gene expressions is still unclear. In this study, the cancer cell lines BGC-823, MKN-45, MKN-28, A549, SPC-A1, and LOVO were exposed to a low-frequency MF. Specific parameters of MFs were determined. Furthermore, the potential of the MF to influence cancer cell growth with midkine (MK) expression was evaluated. Cell proliferation and cell cycle were detected using the CCK-8 assay and flow cytometry. Cell ultrastructure was observed by transmission electron microscopy. BGC-823 cells with over-expression of MK (BGC-MK cells) and stanniocalcin-1 were generated by plasmid construction and transfection. Results showed that exposure to a 0.4-T, 7.5 Hz MF inhibited the proliferation of BGC-823, MKN-28, A549, and LOVO cells, but not MKN-45 and SPC-A1 cells. Moreover, the inhibitory effect of the MF on BGC-MK cells was lower (12.3%) than that of BGC-823 cells (20.3%). Analysis of the cell cycle showed that exposure to the MF led to a significant increase in the S phase in BGC-823 cells, but not in BGC-MK cells. In addition, organelle morphology was modified in BGC-823 cells exposed to the MF. These results suggest that exposure to a 0.4-T, 7.5 Hz MF could inhibit tumor cell proliferation and disturb the cell cycle. The alteration of MK expression in cancer cells may be related to the inhibitory effect of the MF on these cells. Bioelectromagnetics. © 2011 Wiley-Liss, Inc.
Authors:
Tingting Wang; Yunzhong Nie; Shuli Zhao; Yuwang Han; Youwei Du; Yayi Hou
Related Documents :
14617626 - Presentation of galectin-1 by extracellular matrix triggers t cell death.
2264286 - Blastogenic responses and production of tcgf by mononuclear cells isolated from the bov...
16919976 - Establishment and characterization of a spontaneously immortalized porcine mammary epit...
3680956 - Extraction of leucocytes from human decidua. a comparison of dispersal techniques.
19661306 - Selective cytotoxic activities of two novel synthetic drugs on human breast carcinoma m...
22989756 - Rna interference regulates the cell cycle checkpoint through the rna export factor, ptr...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-2-24
Journal Detail:
Title:  Bioelectromagnetics     Volume:  -     ISSN:  1521-186X     ISO Abbreviation:  -     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-3-1     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8008281     Medline TA:  Bioelectromagnetics     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 Wiley-Liss, Inc.
Affiliation:
Immunology and Reproduction Biology Lab, Medical School and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  New computerized fetal heart rate analysis for surveillance of intrauterine growth restriction.
Next Document:  Effects of acetyl salycilic acid and ibuprofen in chronic liver damage induced by CCl(4).