Document Detail


Involvement of microtubule-associated protein 2 (MAP2) in oral cancer cell motility: a novel biological function of MAP2 in non-neuronal cells.
MedLine Citation:
PMID:  18073143     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Microtubule-associated protein 2 (MAP2) has been better known for its well-defined role primarily in neurite outgrowth during neuronal development. However, the biological functions of MAP2 in non-neuronal cells, such as epithelial cells, remain largely unknown. In the present study, we sought to investigate the cellular functions of MAP2 by separately establishing stable expression of two MAP2 isoforms, MAP2A and MAP2C, in oral squamous cell carcinoma, Ca9-22. Ectopic expression of MAP2A or MAP2C results in microtubule bundling predominantly at the cell periphery. Remarkably, overexpression of MAP2A but not MAP2C significantly promotes migration of Ca9-22 cells, whereas knockdown of MAP2A expression by specific siRNA oligos dramatically decreases cell migration of HaCaT, an immortalized keratinocyte cell line with abundant endogenous MAP2A. Furthermore, by immunohistochemical studies, MAP2A was shown to highly and selectively express in invasive oral cancer tissues, consistent with its motility-promoting cellular function revealed through in vitro assays. Thus, our findings have not only identified a novel role of MAP2 in non-neuronal cells, but also provided the first implication of MAP2 in malignant oral cancer tissues.
Authors:
Shyun-Yeu Liu; Yi-Ting Chen; Mei-Yu Tseng; Chih-Chang Hung; Wei-Fan Chiang; Hau-Ren Chen; Tien-Yu Shieh; Chung-Ho Chen; Yuh-Shan Jou; Jeff Yi-Fu Chen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-12-10
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  366     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-01-02     Completed Date:  2008-02-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  520-5     Citation Subset:  IM    
Affiliation:
Department of Oral and Maxillofacial Surgery, Chi-Mei Medical Center, Tainan, Taiwan.
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MeSH Terms
Descriptor/Qualifier:
Cell Line, Tumor
Cell Movement
Humans
Microtubule-Associated Proteins / metabolism*
Mouth Neoplasms / pathology*,  physiopathology*
Neurons / metabolism
Chemical
Reg. No./Substance:
0/Microtubule-Associated Proteins

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