Document Detail


Involvement of mast cells in adipose tissue fibrosis.
MedLine Citation:
PMID:  24326418     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Recently, fibrosis is observed in obese adipose tissue; however, the pathogenesis remains to be clarified. Obese adipose tissue is characterized by chronic inflammation with massive accumulation of immune cells including mast cells. The objective of the present study was to clarify the relationship between fibrosis and mast cells in obese adipose tissue, as well as to determine the origin of infiltrating mast cells. We observed the enhancement of mast cell accumulation and fibrosis in adipose tissue of severely obese diabetic db/db mice. Furthermore, adipose tissue-conditioned medium (ATCM) from severely obese diabetic db/db mice significantly enhanced collagen 5 mRNA expression in NIH-3T3 fibroblasts, and this enhancement was suppressed by the addition of an anti-mast cell protease 6 (MCP-6) antibody. An in vitro study showed that only collagen V among various types of collagen inhibited preadipocyte differentiation. Moreover, we found that ATCM from the nonobese but not obese stages of db/db mice significantly enhanced the migration of bone-marrow-derived mast cells (BMMCs). These findings suggest that immature mast cells that infiltrate into adipose tissue at the nonobese stage gradually mature with the progression of obesity and diabetes, and MCP-6 secreted from mature mast cells induces collagen V expression in obese adipose tissue, which may contribute to the process of adipose tissue fibrosis. Induction of collagen V by MCP-6 might accelerate insulin resistance via the suppression of preadipocyte differentiation.
Authors:
Shizuka Hirai; Chie Ohyane; Young-Il Kim; Shang Lin; Tsuyoshi Goto; Nobuyuki Takahashi; Chu-Sook Kim; Jihey Kang; Rina Yu; Teruo Kawada
Related Documents :
23679988 - Molecular and cellular characterization of buffalo bone marrow-derived mesenchymal stem...
16038708 - Myocardial regeneration by endogenous adult progenitor cells.
23666768 - Multi-potential differentiation of human urine-derived stem cells: potential for therap...
24189428 - Ex-vivo/in-vitro anti-polyethylene glycol (peg) immunoglobulin m production from murine...
15196208 - Compound heterozygosity for pten and ship augments t-dependent humoral immune responses...
19309228 - Epithelial bone marrow cells in patients with advanced esophageal squamous cell carcinoma.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-12-10
Journal Detail:
Title:  American journal of physiology. Endocrinology and metabolism     Volume:  -     ISSN:  1522-1555     ISO Abbreviation:  Am. J. Physiol. Endocrinol. Metab.     Publication Date:  2013 Dec 
Date Detail:
Created Date:  2013-12-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100901226     Medline TA:  Am J Physiol Endocrinol Metab     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
1Kyoto University.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Characterization of an animal model of pregnancy-induced vitamin D deficiency due to metabolic gene ...
Next Document:  Intrinsic circannual regulation of brown adipose tissue form and function in tune with hibernation.