Document Detail

Involvement of ZFPIP/Zfp462 in chromatin integrity and survival of P19 pluripotent cells.
MedLine Citation:
PMID:  20219459     Owner:  NLM     Status:  MEDLINE    
Toti- or pluripotent cells proliferation and/or differentiation have been shown to be strongly related to nuclear chromatin organization and structure over the last past years. We have recently identified ZFPIP/Zfp462 as a zinc finger nuclear factor necessary for correct cell division during early embryonic developmental steps of vertebrates. We thus questioned whether this factor was playing a general role during cell division or if it was somehow involved in embryonic cell fate or differentiation. To achieve this goal, we performed a knock-down experiment in the pluripotent P19 and differentiated 3T3 cell lines, both expressing endogenous ZFPIP/Zfp462. Using specific shRNA directed against ZFPIP/Zfp462 transcripts, we demonstrated that depletion of this protein induced cell death in P19 but had no effect in 3T3 cells. In addition, in the absence of the protein, the P19 cells exhibited a complete destructuration of pericentromeric domains associated with a redistribution of the HP1alpha proteins and an increase in DNA satellites transcribed RNAs level. These data suggested an instrumental role of ZFPIP/Zfp462 in maintaining the chromatin structure of pluripotent cells.
Julie Mass?; Audrey Laurent; Barbara Nicol; Daniel Guerrier; Isabelle Pellerin; St?phane Deschamps
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-02-26
Journal Detail:
Title:  Experimental cell research     Volume:  316     ISSN:  1090-2422     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-04-05     Completed Date:  2010-05-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1190-201     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier Inc. All rights reserved.
UMR CNRS 6061, Institut of G?n?tique et D?veloppement de Rennes (IGDR), Facult? de M?decine, Universit? de Rennes 1, Rennes, France.
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MeSH Terms
3T3 Cells
Apoptosis / drug effects,  genetics
COS Cells
Carrier Proteins / physiology*
Cell Division / drug effects
Cell Survival / drug effects,  genetics
Cells, Cultured
Cercopithecus aethiops
Chromatin / chemistry,  genetics,  metabolism*
Chromosomal Instability / genetics,  physiology
Embryo, Mammalian
Gene Expression Regulation / drug effects
Nerve Tissue Proteins / physiology*
Pluripotent Stem Cells / drug effects,  metabolism,  physiology*
RNA, Small Interfering / pharmacology
Reg. No./Substance:
0/Carrier Proteins; 0/Chromatin; 0/Nerve Tissue Proteins; 0/RNA, Small Interfering; 0/Zfp462 protein, mouse

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