| Involvement of ZFPIP/Zfp462 in chromatin integrity and survival of P19 pluripotent cells. | |
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MedLine Citation:
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PMID: 20219459 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Toti- or pluripotent cells proliferation and/or differentiation have been shown to be strongly related to nuclear chromatin organization and structure over the last past years. We have recently identified ZFPIP/Zfp462 as a zinc finger nuclear factor necessary for correct cell division during early embryonic developmental steps of vertebrates. We thus questioned whether this factor was playing a general role during cell division or if it was somehow involved in embryonic cell fate or differentiation. To achieve this goal, we performed a knock-down experiment in the pluripotent P19 and differentiated 3T3 cell lines, both expressing endogenous ZFPIP/Zfp462. Using specific shRNA directed against ZFPIP/Zfp462 transcripts, we demonstrated that depletion of this protein induced cell death in P19 but had no effect in 3T3 cells. In addition, in the absence of the protein, the P19 cells exhibited a complete destructuration of pericentromeric domains associated with a redistribution of the HP1alpha proteins and an increase in DNA satellites transcribed RNAs level. These data suggested an instrumental role of ZFPIP/Zfp462 in maintaining the chromatin structure of pluripotent cells. |
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Authors:
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Julie Mass?; Audrey Laurent; Barbara Nicol; Daniel Guerrier; Isabelle Pellerin; St?phane Deschamps |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-02-26 |
Journal Detail:
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Title: Experimental cell research Volume: 316 ISSN: 1090-2422 ISO Abbreviation: Exp. Cell Res. Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-04-05 Completed Date: 2010-05-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0373226 Medline TA: Exp Cell Res Country: United States |
Other Details:
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Languages: eng Pagination: 1190-201 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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UMR CNRS 6061, Institut of G?n?tique et D?veloppement de Rennes (IGDR), Facult? de M?decine, Universit? de Rennes 1, Rennes, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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3T3 Cells Animals Apoptosis / drug effects, genetics COS Cells Carrier Proteins / physiology* Cell Division / drug effects Cell Survival / drug effects, genetics Cells, Cultured Cercopithecus aethiops Chromatin / chemistry, genetics, metabolism* Chromosomal Instability / genetics, physiology Embryo, Mammalian Gene Expression Regulation / drug effects Mice Nerve Tissue Proteins / physiology* Pluripotent Stem Cells / drug effects, metabolism, physiology* RNA, Small Interfering / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/Chromatin; 0/Nerve Tissue Proteins; 0/RNA, Small Interfering; 0/Zfp462 protein, mouse |
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