Document Detail


Involvement of the choroid plexus in the inflammatory response after acute spinal cord injury in dogs: an immunohistochemical study.
MedLine Citation:
PMID:  22840733     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The choroid plexus (CP) is increasingly recognized as an important contributor to central nervous system (CNS) inflammation by recruitment of inflammatory cells and release of inflammatory cytokines. Here we investigate the role of the CP epithelium (CPE) as a source of three pro-inflammatory molecules of potential importance in inflammation after acute spinal cord injury (SCI): IL-1β, TNF-α, and hsp70. Immunohistochemical (IHC) staining for these three proteins was performed on 4th ventricular CPE from 4 dogs euthanized 12-48 h after spontaneous acute SCI, and from 4 neurologically normal dogs euthanized for other reasons. IHC staining was quantified using Aperio ImageScope software. IHC staining in the CPE of dogs with acute SCI was 2.2, 1.6 and 1.5 times higher than that of normal dogs, for IL-1β, TNF-α, and hsp70, respectively. Increases were statistically significant (p<0.1) for IL-1β and TNF-α, and closely approached significance for hsp70. These findings indicate that the CPE could serve as an important source of these inflammatory mediators after SCI. There was also an inverse correlation between IL-1β and hsp70 staining and duration of clinical signs in acute SCI, suggesting that increased expression of these proteins by the CPE may be of particular importance in the immediate-early inflammatory response after acute SCI.
Authors:
Sarah A Moore; Michael J Oglesbee
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-07-13
Journal Detail:
Title:  Veterinary immunology and immunopathology     Volume:  148     ISSN:  1873-2534     ISO Abbreviation:  Vet. Immunol. Immunopathol.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-08-13     Completed Date:  2013-01-07     Revised Date:  2014-11-06    
Medline Journal Info:
Nlm Unique ID:  8002006     Medline TA:  Vet Immunol Immunopathol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  348-52     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier B.V. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Animals
Choroid Plexus / chemistry,  immunology,  pathology,  physiopathology*
Dogs
HSP70 Heat-Shock Proteins / analysis
Inflammation / immunology,  physiopathology,  veterinary
Interleukin-1beta / analysis
Spinal Cord Injuries / immunology,  pathology,  physiopathology,  veterinary*
Tumor Necrosis Factor-alpha / analysis
Grant Support
ID/Acronym/Agency:
P30 CA016058/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/HSP70 Heat-Shock Proteins; 0/Interleukin-1beta; 0/Tumor Necrosis Factor-alpha

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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