| Involvement of caspases and apoptosis-inducing factor in bufotalin-induced apoptosis of Hep 3B cells. | |
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MedLine Citation:
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PMID: 19055367 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bufotalin is one of the bufadienolides isolated from Formosan Ch'an Su, which is made of the skin and parotid glands of toads. Ingestion of toad venom results in severe morbidity and high mortality. Although Ch'an Su is clinically toxic, it has been used as an important traditional Chinese medicine for heart failure and pains. In this study, bufotalin-induced apoptosis in human hepatocellular carcinoma Hep 3B cells was investigated. The results indicate that externalization of phosphatidylserine, accumulation of sub-G(1) cells, fragmentation of DNA, and formation of apoptotic bodies were observed in bufotalin-treated Hep 3B cells. The signaling pathway might be via the activation of caspase-8, increase in mitochondrial tBid, disruption of mitochondrial membrane potential, and translocation of apoptosis-inducing factor (AIF). Active caspase-8 might activate caspase-9 and caspase-3 leading to the cleavage of nuclear PARP. Presence of AIF and cleaved PARP in the nuclei might lead to DNA fragmentation. Caspase-8 inhibitor (Z-IETD) or wide-ranging caspase inhibitor (Z-VAD) significantly suppressed the bufotalin-induced apoptosis, while the anti-Fas neutralization antibody had no effect. These data suggest that bufotalin-induced apoptosis in Hep 3B cells might involve caspases and AIF. |
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Authors:
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Chun-Li Su; Ting-Yu Lin; Chun-Nan Lin; Shen-Jeu Won |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of agricultural and food chemistry Volume: 57 ISSN: 1520-5118 ISO Abbreviation: J. Agric. Food Chem. Publication Date: 2009 Jan |
Date Detail:
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Created Date: 2009-01-07 Completed Date: 2009-02-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: United States |
Other Details:
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Languages: eng Pagination: 55-61 Citation Subset: IM |
Affiliation:
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Department of Nursing, Chang Jung Christian University, Gueiren, Tainan 711, Taiwan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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drug effects,
physiology* Apoptosis Inducing Factor / physiology* Bufanolides / pharmacology* Carcinoma, Hepatocellular Caspase 8 / metabolism Caspases / metabolism* Cell Line, Tumor Humans Liver Neoplasms Signal Transduction |
| Chemical | |
Reg. No./Substance:
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0/Apoptosis Inducing Factor; 0/Bufanolides; 471-95-4/bufotalin; EC 3.4.22.-/Caspase 8; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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