Document Detail


Involvement of calpain in the process of Jurkat T cell chemotaxis.
MedLine Citation:
PMID:  18831007     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Massive T cell infiltration into the central nervous system is a hallmark of multiple sclerosis (MS) and its rodent model experimental autoimmune encephalomyelitis (EAE), resulting in the induction of many of the pathophysiological events that lead to neuroinflammation and neurodegeneration. Thus, blocking T cell migration into the central nervous system may reduce disease severity in MS and EAE. One potential target for reducing T cell migration is inhibition of the Ca(2+)-activated neutral protease calpain. Previous studies in other cell types have demonstrated that migration is reduced by incubation of cells with calpain inhibitors. Thus, we hypothesize that calpain inhibition will reduce migration of T cells in response to and toward the chemokine CCL2. To test this hypothesis, the intracellular free Ca(2+) levels in Jurkat E6-1 T cells was first measured by the fura-2 assay to assess whether the intracellular ion environment would support calpain activation. The intracellular free Ca(2+) levels were found to increase in response to CCL2. The cells were next treated with the calpain inhibitor calpeptin in a multiwelled Boyden chamber with CCL2 used as the chemoattractant. These studies demonstrate that inhibition of calpain with its inhibitor calpeptin produces a dose-dependent inhibition of chemotaxis. Calpain activity, as measured by live cell imaging, was also increased in response to CCL2, providing further evidence of its involvement in the process of chemotaxis and migration. These studies provide evidence for the involvement of calpain in the mechanisms of chemotaxis and warrants further exploration in MS patient and EAE animal samples.
Authors:
Jonathan T Butler; Supriti Samantaray; Craig C Beeson; Swapan K Ray; Naren L Banik
Related Documents :
8779937 - Mechanical measurements from isolated cardiac myocytes using a pipette attachment system.
9748497 - Radiation-induced long-lived radicals which cause mutation and transformation.
14403807 - The morphology and behavior of living exoerythrocytic stages of plasmodium gallinaceum ...
16204347 - Oxfordgrid: a web interface for pairwise comparative map views.
16254827 - Embelin derivatives and their anticancer activity through microtubule disassembly.
7537687 - Growth regulation and cellular changes during differentiation of human prostatic cancer...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Journal of neuroscience research     Volume:  87     ISSN:  1097-4547     ISO Abbreviation:  J. Neurosci. Res.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-02-03     Completed Date:  2009-03-31     Revised Date:  2014-09-20    
Medline Journal Info:
Nlm Unique ID:  7600111     Medline TA:  J Neurosci Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  626-35     Citation Subset:  IM    
Copyright Information:
(c) 2008 Wiley-Liss, Inc.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Calcium / metabolism
Calpain / antagonists & inhibitors,  metabolism*
Chemokine CCL2 / pharmacology
Chemotaxis, Leukocyte / physiology*
Cysteine Proteinase Inhibitors / pharmacology
Dipeptides / pharmacology
Dose-Response Relationship, Drug
Fura-2
Glycoproteins / pharmacology
Humans
Jurkat Cells
Signal Transduction / physiology
T-Lymphocytes / drug effects,  physiology*
Grant Support
ID/Acronym/Agency:
ES-12878/ES/NIEHS NIH HHS; NS-31622/NS/NINDS NIH HHS; NS-41088/NS/NINDS NIH HHS; NS-45967/NS/NINDS NIH HHS; NS-56176/NS/NINDS NIH HHS; NS-57811/NS/NINDS NIH HHS; R01 NS041088/NS/NINDS NIH HHS; R01 NS041088-06/NS/NINDS NIH HHS; R01 NS041088-07/NS/NINDS NIH HHS; R01 NS041088-08/NS/NINDS NIH HHS; R01 NS056176/NS/NINDS NIH HHS; R01 NS056176-03/NS/NINDS NIH HHS; R01 NS056176-04/NS/NINDS NIH HHS; R01 NS057811-02/NS/NINDS NIH HHS; R01 NS057811-03/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Chemokine CCL2; 0/Cysteine Proteinase Inhibitors; 0/Dipeptides; 0/Glycoproteins; 0/calpain inhibitors; 117591-20-5/calpeptin; EC 3.4.22.-/Calpain; SY7Q814VUP/Calcium; TSN3DL106G/Fura-2
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Entrainment and coupling of the hamster suprachiasmatic clock by daily dark pulses.
Next Document:  Ultrastructural and temporal changes of the microvascular basement membrane and astrocyte interface ...