Document Detail

Involvement of calcitonin gene-related peptide in the modulation of human myometrial contractility during pregnancy.
MedLine Citation:
PMID:  10487770     Owner:  NLM     Status:  MEDLINE    
Calcitonin gene-related peptide (CGRP) is a potent vasodilator and relaxes smooth muscle of a variety of tissues, but the effects of CGRP on human myometrial contractions and the changes in CGRP receptors (CGRP-Rs) in human myometrium have not been described. We report that CGRP induced dose-dependent relaxation in spontaneously contracting myometrium from pregnant women. This relaxation effect is diminished in myometrium obtained from patients during labor and in the nonpregnant state. CGRP-induced relaxations are inhibited by a CGRP-R antagonist (CGRP(8-37)), a soluble guanylate cyclase inhibitor (LY(83583)), and a nitric oxide synthase inhibitor (L-NAME). Both Western blotting and mRNA analysis showed that CGRP-Rs are present in human myometrium, and that the expression of these receptors is increased during pregnancy and decreased during term labor. Immunofluorescent staining revealed that CGRP-Rs are abundant in the myometrial cells of pregnant women who are not in labor, and are minimal in uterine specimens from women in labor and in the nonpregnant state. We conclude that increased CGRP-Rs in myometrium, and resulting enhanced myometrial sensitivity to CGRP, may play a role in maintaining human myometrium in a quiescent state during pregnancy, and that a decline in the CGRP-Rs at term could contribute to the initiation of labor.
Y L Dong; L Fang; S Kondapaka; P R Gangula; S J Wimalawansa; C Yallampalli
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of clinical investigation     Volume:  104     ISSN:  0021-9738     ISO Abbreviation:  J. Clin. Invest.     Publication Date:  1999 Sep 
Date Detail:
Created Date:  1999-10-04     Completed Date:  1999-10-04     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  7802877     Medline TA:  J Clin Invest     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  559-65     Citation Subset:  AIM; IM    
Department of Obstetrics and Gynecology, University of Texas Medical Branch, Galveston, Texas 77555, USA.
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MeSH Terms
Aminoquinolines / pharmacology
Calcitonin Gene-Related Peptide / pharmacology,  physiology*
Cesarean Section
Cyclic GMP / physiology
Enzyme Inhibitors / pharmacology
Guanylate Cyclase / antagonists & inhibitors
Myometrium / drug effects,  physiology*
NG-Nitroarginine Methyl Ester / pharmacology
Nitric Oxide Synthase / antagonists & inhibitors
Peptide Fragments / pharmacology
Pregnancy / physiology*
Reverse Transcriptase Polymerase Chain Reaction
Single-Blind Method
Uterine Contraction / drug effects,  physiology*
Grant Support
Reg. No./Substance:
0/Aminoquinolines; 0/Enzyme Inhibitors; 0/Peptide Fragments; 119911-68-1/calcitonin gene-related peptide (8-37); 50903-99-6/NG-Nitroarginine Methyl Ester; 7665-99-8/Cyclic GMP; 83652-28-2/Calcitonin Gene-Related Peptide; 91300-60-6/6-anilino-5,8-quinolinedione; EC Oxide Synthase; EC Cyclase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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