Document Detail

Involvement of apoptosis and proliferation of acinar cells in atrophy of rat parotid glands induced by liquid diet.
MedLine Citation:
PMID:  22918835     Owner:  NLM     Status:  MEDLINE    
Parotid glands of experimental animals fed a liquid diet are reported to show atrophy (Hall and Schneyer 1964; Wilborn and Schneyer 1970; Hand and Ho 1981; Scott et al. 1990; Scott and Gunn 1991). To clarify whether apoptosis and proliferation of acinar cells participate in atrophy of rat parotid glands induced by liquid diet, rats were fed a liquid diet and compared to pellet-fed controls. Parotid glands were removed at 3, 7, 14 or 21 days, weighed, and examined using transmission electron microscopy (TEM), and studied immunohistochemically for cleaved-caspase-3 (Casp-3), a marker of apoptotic cells, and 5-bromo-2'-deoxyuridine (BrdU), a marker for proliferating cells. Body weights of experimental rats fed liquid diets were not significantly different from controls fed pellet diets; however weights of experimental parotid glands were smaller than those of controls. In the experimental parotid glands, structures like apoptotic bodies were histologically observed in acini at each time point; more Casp-3-positive acinar cells were identified in experimental parotid glands than in the controls on days 3, 7, and 14. Experimental glands showed fewer BrdU-positive acinar cells at each time point. TEM confirmed typical apoptotic acinar cells in the atrophic glands. These findings suggest that increased acinar cell apoptosis and reduced acinar cell proliferation occur in atrophic parotid glands of rats fed a liquid diet.
Shigeru Takahashi; Hiroki Uekita; Tsuyoshi Kato; Fumihiko Yuge; Natsumi Ushijima; Kiichiro Inoue; Takanori Domon
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-08-24
Journal Detail:
Title:  Journal of molecular histology     Volume:  43     ISSN:  1567-2387     ISO Abbreviation:  J. Mol. Histol.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-27     Completed Date:  2013-05-03     Revised Date:  2013-07-10    
Medline Journal Info:
Nlm Unique ID:  101193653     Medline TA:  J Mol Histol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  761-6     Citation Subset:  IM    
Department of Oral Functional Anatomy, Hokkaido University Graduate School of Dental Medicine, Kita 13, Nishi 7, Kita-ku, Sapporo, 060-8586, Japan.
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MeSH Terms
Apoptosis / physiology*
Cell Proliferation
Microscopy, Electron, Transmission
Parotid Gland / cytology*,  ultrastructure

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