Document Detail


Involvement of the annexin II-S100A10 complex in the formation of E-cadherin-based adherens junctions in Madin-Darby canine kidney cells.
MedLine Citation:
PMID:  15574423     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
E-cadherin and nectins are major cell-cell adhesion molecules at adherens junctions (AJs) in epithelial cells. When Madin-Darby canine kidney (MDCK) cells stably expressing nectin-1 (nectin-1-MDCK cells) are cultured at normal Ca(2+), E-cadherin and nectin-1 are concentrated at the cell-cell contact sites. When these cells are cultured at low Ca(2+), E-cadherin disappears from the cell-cell contact sites, but nectin-1 persists there. When these cells are re-cultured at normal Ca(2+), E-cadherin is recruited to the nectin-based cell-cell contact sites. We found here that this recruitment was dependent on protein synthesis, because a protein synthesis inhibitor, cycloheximide, prevented the accumulation of E-cadherin. When nectin-1-MDCK cells, precultured at low Ca(2+) in the presence of a proteasome inhibitor, ALLN (N-acetyl-Leu-Leu-norleucinal), were re-cultured at normal Ca(2+), E-cadherin was recruited to the nectin-based cell-cell contact sites but the level of E-cadherin was reduced. Similar results were obtained when wild-type MDCK cells were used instead of nectin-1-MDCK cells. These results suggest that degradation of one or more protein factors and de novo synthesis of the same or different protein factor(s) are needed for the formation of the E-cadherin-based AJs. We biochemically identified the annexin II-S100A10 complex as such a candidate. Depletion of plasma membrane cholesterol, which abolished the localization of the annexin II-S100A10 complex at the plasma membrane, inhibited the re-concentration of E-cadherin at the nectin-based cell-cell contact sites in the Ca(2+) switch experiment. Knockdown of annexin II by RNA interference also inhibited the re-concentration of E-cadherin. These results indicate that the annexin II-S100A10 complex is involved in the formation of the E-cadherin-based AJs in MDCK cells.
Authors:
Akio Yamada; Kenji Irie; Takeshi Hirota; Takako Ooshio; Atsunori Fukuhara; Yoshimi Takai
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-12-01
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  280     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2005-02-14     Completed Date:  2005-04-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6016-27     Citation Subset:  IM    
Affiliation:
Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine/Faculty of Medicine, Suita 565-0871, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adherens Junctions / metabolism*
Animals
Annexin A2 / genetics,  metabolism*
Binding Sites
Biotinylation
Cadherins / genetics,  metabolism*
Calcium / metabolism,  pharmacology
Cell Adhesion
Cell Adhesion Molecules / metabolism
Cell Line
Cell Membrane / chemistry,  drug effects,  metabolism
Cholesterol / metabolism
Cycloheximide / pharmacology
Dogs
Kidney / cytology*,  metabolism*
RNA Interference
S100 Proteins / metabolism*
Chemical
Reg. No./Substance:
0/Annexin A2; 0/Cadherins; 0/Cell Adhesion Molecules; 0/S100 Proteins; 0/S100 calcium binding protein A10; 0/nectins; 57-88-5/Cholesterol; 66-81-9/Cycloheximide; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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