| Involvement of alpha v beta 3 integrin in mediating fibrin gel retraction. | |
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MedLine Citation:
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PMID: 7530248 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Platelet integrin alpha IIb beta 3 (GPIIb-IIIa) plays important roles in platelet-mediated clot retraction. However, little is known about the mechanisms of clot retraction mediated by nucleated cells. In this report, we demonstrate that another member of the beta 3 integrin family, alpha v beta 3, is involved in clot retraction mediated by nucleated cells. Retraction of fibrin clots was observed using a human melanoma cell line, C32TG, which contains no alpha IIb beta 3 complex. This retraction was inhibited by RGD-containing peptide, monoclonal anti-beta 3, and anti-alpha v beta 3 antibodies. Immunoelectron microscopic studies revealed a direct interaction between beta 3 integrin and fibrin fibers at an early stage of clot retraction. We found that another human embryonal cell line, 293, which is known to express alpha v beta 1, but no alpha v beta 3, lacks fibrin gel retractile activity. Upon transfection of beta 3 DNA into 293 cells, the beta 3 subunit formed a complex with an endogenous alpha v subunit. The beta 3-bearing transfectants were found to retract fibrin gels, which was specifically inhibited by anti-beta 3 antibody. In addition, a point mutation at Asp119 in the beta 3 ligand binding domain abolished the clot retractile activity of 293 transfectants, indicating the requirement of alpha v beta 3 ligand-binding activity. Our findings suggest that alpha v beta 3 is involved in mediating the interaction between the three-dimensional fibrin network and nucleated cells and in promoting "post-receptor occupancy" events. |
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Authors:
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Y Katagiri; T Hiroyama; N Akamatsu; H Suzuki; H Yamazaki; K Tanoue |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 270 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1995 Jan |
Date Detail:
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Created Date: 1995-02-22 Completed Date: 1995-02-22 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1785-90 Citation Subset: IM |
Affiliation:
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Department of Cardiovascular Research, Tokyo Metropolitan Institute of Medical Science, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antibodies, Monoclonal Blood Platelets / drug effects, physiology* Cell Line Clot Retraction* Embryo, Mammalian Fibrin / drug effects, physiology*, ultrastructure Humans Integrins / physiology* Melanoma Microscopy, Electron Oligopeptides / pharmacology Platelet Glycoprotein GPIIb-IIIa Complex Platelet Membrane Glycoproteins / antagonists & inhibitors Receptors, Cytoadhesin / physiology* Receptors, Vitronectin Recombinant Proteins / metabolism Transfection Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Integrins; 0/Oligopeptides; 0/Platelet Glycoprotein GPIIb-IIIa Complex; 0/Platelet Membrane Glycoproteins; 0/Receptors, Cytoadhesin; 0/Receptors, Vitronectin; 0/Recombinant Proteins; 138107-58-1/Ro 43-5054; 9001-31-4/Fibrin; 91037-75-1/glycyl-arginyl-glycyl-aspartyl-seryl-proline; 99896-88-5/glycyl-arginyl-glycyl-glutamyl-seryl-proline |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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